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Zeinali, Khadijeh, Khorasani, Mohammad Taghi, Ataie, Maryam. (1404). Cytocompatibility of a 3- Dimensional Graphene Oxide/ Laminin Composite Scaffold Intended for Nerve Tissue Engineering. , 58(2), 264-276. doi: 10.22059/jufgnsm.2025.02.11
Khadijeh Zeinali; Mohammad Taghi Khorasani; Maryam Ataie. "Cytocompatibility of a 3- Dimensional Graphene Oxide/ Laminin Composite Scaffold Intended for Nerve Tissue Engineering". , 58, 2, 1404, 264-276. doi: 10.22059/jufgnsm.2025.02.11
Zeinali, Khadijeh, Khorasani, Mohammad Taghi, Ataie, Maryam. (1404). 'Cytocompatibility of a 3- Dimensional Graphene Oxide/ Laminin Composite Scaffold Intended for Nerve Tissue Engineering', , 58(2), pp. 264-276. doi: 10.22059/jufgnsm.2025.02.11
Zeinali, Khadijeh, Khorasani, Mohammad Taghi, Ataie, Maryam. Cytocompatibility of a 3- Dimensional Graphene Oxide/ Laminin Composite Scaffold Intended for Nerve Tissue Engineering. , 1404; 58(2): 264-276. doi: 10.22059/jufgnsm.2025.02.11

Cytocompatibility of a 3- Dimensional Graphene Oxide/ Laminin Composite Scaffold Intended for Nerve Tissue Engineering

Journal of Ultrafine Grained and Nanostructured Materials
مقاله 11، دوره 58، شماره 2، اسفند 2025، صفحه 264-276    اصل مقاله (1.09 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22059/jufgnsm.2025.02.11
نویسندگان
Khadijeh Zeinali1؛ Mohammad Taghi Khorasani2؛ Maryam Ataie* 3
1Food and Drug Research Center, Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran
2Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, Iran
3Faculty of Life Science Engineering, College of Interdisciplinary Science and technology, University of Tehran, Tehran, Iran
چکیده
In this study, we fabricated porous and low-molecular-weight graphene oxide (GO) aerogel scaffolds via a single-step chemical reduction of GO with ethylene diamine (EDA) at 95°C for 6 hours, followed by freeze-drying. The concentrations of GO (3–8 mg/ml) and EDA (20–50 µl) were optimized using Design of Experiments (DOE) to control scaffold density and porosity. The scaffold featuring 5 mg/ml GO and 30 µl EDA exhibited optimal porous architecture with interconnected pores visible in SEM analysis and a low density of approximately 13 mg/cm³. Laminin coating was applied to enhance biological functionality.
Biocompatibility was confirmed using MTT assays with L929 fibroblast cells, showing 124% cell viability after 72 hours, indicating no cytotoxicity and even promotion of cell proliferation. P19 mouse embryonal carcinoma cells cultured on these scaffolds successfully differentiated into neural cells, as evidenced by the expression of microtubule-associated protein-2 (MAP-2) confirmed via immunofluorescence staining. Quantitatively, neural differentiation efficiency on the scaffold reached approximately 80%, significantly higher compared to controls.
These results demonstrate that the developed GO aerogel scaffold possesses suitable mechanical properties, porosity, and bioactivity to support neural cell adhesion, proliferation, and differentiation. The scaffold’s tunable density and porosity via GO and EDA concentrations provide a controllable microenvironment for neural tissue engineering applications. Future work will explore in vivo regenerative potential and electrical conductivity integration to further enhance nerve tissue repair.
کلیدواژه‌ها
Graphene oxide aerogel؛ Neural tissue engineering؛ Scaffold fabrication؛ Cell differentiation؛ Biocompatibility
مراجع
 

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