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Priosoeryanto, Bambang, Amalia, Fhatania, Purwono, Rini, Prasetyo, Bayu, Pertiwi, Mega, Amira, Urfa, Melati, Alfriyan. (1404). In Silico and In Vitro Anticancer Activities of Punica granatum L Peel Ethanolic Extract Against Topoisomerase II Alpha in HeLa and cMM/IPB-B4 Cell Lines. , (), -. doi: 10.22059/ijvm.2025.400986.1005882
Bambang Pontjo Priosoeryanto; Fhatania Amalia; Rini Madyastuti Purwono; Bayu Febram Prasetyo; Mega Sonia Pertiwi; Urfa Amira; Alfriyan Krisna Melati. "In Silico and In Vitro Anticancer Activities of Punica granatum L Peel Ethanolic Extract Against Topoisomerase II Alpha in HeLa and cMM/IPB-B4 Cell Lines". , , , 1404, -. doi: 10.22059/ijvm.2025.400986.1005882
Priosoeryanto, Bambang, Amalia, Fhatania, Purwono, Rini, Prasetyo, Bayu, Pertiwi, Mega, Amira, Urfa, Melati, Alfriyan. (1404). 'In Silico and In Vitro Anticancer Activities of Punica granatum L Peel Ethanolic Extract Against Topoisomerase II Alpha in HeLa and cMM/IPB-B4 Cell Lines', , (), pp. -. doi: 10.22059/ijvm.2025.400986.1005882
Priosoeryanto, Bambang, Amalia, Fhatania, Purwono, Rini, Prasetyo, Bayu, Pertiwi, Mega, Amira, Urfa, Melati, Alfriyan. In Silico and In Vitro Anticancer Activities of Punica granatum L Peel Ethanolic Extract Against Topoisomerase II Alpha in HeLa and cMM/IPB-B4 Cell Lines. , 1404; (): -. doi: 10.22059/ijvm.2025.400986.1005882

In Silico and In Vitro Anticancer Activities of Punica granatum L Peel Ethanolic Extract Against Topoisomerase II Alpha in HeLa and cMM/IPB-B4 Cell Lines

Iranian Journal of Veterinary Medicine
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 20 آبان 1404
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22059/ijvm.2025.400986.1005882
نویسندگان
Bambang Pontjo Priosoeryanto* 1؛ Fhatania Amalia2؛ Rini Madyastuti Purwono3؛ Bayu Febram Prasetyo3؛ Mega Sonia Pertiwi4؛ Urfa Amira5؛ Alfriyan Krisna Melati5
1Division of Veterinary Pathology , School of Veterinary Medicine and Biomedical Sciences, IPB University
2Animal Biomedical Science Study Program, School of Veterinary Medicine and Biomedical Sciences, IPB University
3Division of Veterinary Pharmacology, School of Veterinary Medicine and Biomedical Sciences, IPB University
4Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
5School of Veterinary Medicine and Biomedical Sciences, IPB University
چکیده
Background: Cancer remains a leading cause of death globally, and the development of new, effective, and less toxic treatments from natural sources is a key area of research. Pomegranate peel, a rich source of bioactive compounds, has shown promise as a natural anticancer agent.
Objectives: This study examines the anti-proliferative properties of pomegranate peel ethanolic extract against cMM/IPB-B4 and HeLa cells. The study also utilized in silico approaches to explore the potential inhibitory activities of the pomegranate’s major compounds, such as punicalagin, ellagic acid, and gallic acid, against Topoisomerase IIα.
Methods: An ethanolic extract of pomegranate peel was prepared using maceration method. In vitro anti-proliferative activity was assessed using a cell viability assay on HeLa and cMM/IPB-B4 cells. The IC50 values were determined, and a BSLT was conducted to evaluate toxicity. An in silico analysis using AutoDock Vina, PASS Online, ADMET-AI screening was performed to study the interactions of the compounds with the Topoisomerase IIα enzyme.
Results: The extract demonstrated anti-proliferative effects with IC50 values of 943.43 µg/mL for HeLa cells and 1152.42 µg/mL for cMM/IPB-B4 cells. In silico docking revealed that punicalagin is the best Topoisomerase IIα inhibitor (-7.24 kcal/mol). The ADMET analysis indicated that punicalagin has a moderately safety profile.
Conclusion: Pomegranate peel ethanolic extract exhibits anti-proliferative activity against both HeLa and cMM/IPB-B4 cells. The in silico findings strongly suggest that punicalagin is a key compound contributing to this effect by inhibiting Topoisomerase IIα. These results support the potential of pomegranate peel extract as novel anticancer therapies, especially against cMM/IPB-B4.
کلیدواژه‌ها
Apoptosis؛ Mesothelioma؛ Molecular Docking؛ Pomegranate؛ TopoIIα
آمار
تعداد مشاهده مقاله: 144





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