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Production of parental lines using temperature and radiation treatments in greenhouse cucumber (Cucumis sativus L.) | ||
| International Journal of Horticultural Science and Technology | ||
| دوره 13، شماره 2، تیر 2026، صفحه 293-304 اصل مقاله (622.22 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.22059/ijhst.2025.384440.947 | ||
| نویسندگان | ||
| Sibgol Khoshkam* 1؛ Davood Samsampour2؛ Mehran Enayati Shariat Panahi3؛ Behnam Naserian Khiabani4 | ||
| 1PhD student, Department of Agricultural Engineering, Faculty of Agriculture and Natural Resources, University of Hormozgan, Hormozgan, Iran | ||
| 2Associate Professor, Department of Agricultural Engineering, Faculty of Agriculture and Natural Resources, Hormozgan University, Hormozgan, Iran | ||
| 3Professor of the Department of Culture, Tissue and Cells, Agricultural Biotechnology Research Institute, Agricultural Research, Education and Extension Organization, Iran | ||
| 4Researcher of Radiation Application Research Institute, Nuclear Science and Technology Research Institute, Atomic Energy Organization, Iran | ||
| چکیده | ||
| Haploid and double-haploid technology through gametophytic embryogenesis and the altered expression of genes involved in the emergence of important agricultural traits allows for utilizing the genetic potential of genotypes and provides a suitable strategy for hybrid seed production. To this aim, this research was conducted in two two-factor factorial experiments as a completely randomized design with three replications in the research greenhouses of the Southern Agricultural and Natural Resources Research Center of Kerman province in 2023. Five levels of genotypes were determined as the first factor, and pollen radiation at two doses (300 and 350 Gy) and two temperature treatments of 35 °C for 6 and 12 hours to sterilize greenhouse cucumber pollens were specified as the second factor. The results indicated that haploid seedlings were obtained through parthenogenesis induction in cucumber genotypes. One-third of the fruits after pollination with treated pollens contained at least one parthenogenetic embryo. Since an optimal radiation dose is a key influential factor on the efficiency of parthenogenetic embryo induction, the ploidy level in double-haploid seedlings treated with 300 Gy of gamma radiation (10.67) and 6-hour temperature treatment at 35 °C (8.68) led to the highest haploid induction. Half of the produced plants possessed the haploid chromosome number and a combination of normal chromosomes and mixoploid chromosomes. The chromosome number was doubled in haploid plants by the laboratory colchicine treatment at a concentration of 500 mg/l for 24 h. This method can be used to produce homozygous lines for use in cucumber breeding programs. | ||
| کلیدواژهها | ||
| Chromosome؛ Cucurbitaceae؛ Hybrid seed؛ Haploid؛ Induction of parthenocarpy؛ Treated pollen | ||
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آمار تعداد مشاهده مقاله: 328 تعداد دریافت فایل اصل مقاله: 227 |
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