| تعداد نشریات | 127 |
| تعداد شمارهها | 7,120 |
| تعداد مقالات | 76,523 |
| تعداد مشاهده مقاله | 152,945,003 |
| تعداد دریافت فایل اصل مقاله | 115,098,765 |
تولید گیاهان زعفران عاری از ویروس پنهان زعفران با بکارگیری کشت مریستم و گرمادرمانی | ||
| دانش گیاهپزشکی ایران | ||
| دوره 55، شماره 2، اسفند 1403، صفحه 331-348 اصل مقاله (1.79 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijpps.2025.396499.1007077 | ||
| نویسندگان | ||
| مریم رحیمی1؛ سیامک کلانتری* 2؛ اکبر دیزجی3؛ مجید شکرپور4 | ||
| 1گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تهران، کرج- ایران | ||
| 2گروه علوم باغبانی، دانشکده کشاورزی ی، دانشگاه تهران ، کرج- ایران | ||
| 3گروه گیاهپزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
| 4گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تهران، کرج - ایران | ||
| چکیده | ||
| زعفران (Crocus sativus L.) یکی از مهمترین گیاهان دارویی و ادویهای در سراسر جهان است. این گیاه منبع غنی آپوکاروتنوئیدها مانند استرهای کروستین، پیکروکروسین و سافرانال است. ویروس پنهان زعفران (saffron latent virus; SaLV)، از جنس Potyvirus و خانواده Potyviridae، شایعترین ویروس شناختهشده زعفران در ایران است که بیش از 70 درصد گیاهان زعفران کشور را در مزارع آلوده نموده است. به منظور ویروسزدایی گیاهان آلوده زعفران از SaLV، در پژوهش حاضر دو روش کشت مریستم (با اندازه مریستم 1/0، 3/0، 5/0 و 7/0 میلیمتر) و گرمادرمانی به کار برده شد. برای ردیابی ویروس از آزمون سرولوژی ELISA-ACP و مولکولی RT-PCR و RT-qPCR استفاده شد. نتایج نشان داد که بیشترین درصد گیاهان عاری از ویروس بر اساس آزمون الایزا و RT-PCR، به ترتیب با 73/83 و 29/81 درصد در تیمار انفرادی کشت مریستم با اندازه 3/0 میلیمتر و به ترتیب با 36/71 و 36/66 درصد در تیمار انفرادی گرمادرمانی 50 درجه سلسیوس به مدت 60 دقیقه به دست آمد. بیشترین درصد گیاهان عاری از ویروس بر اساس آزمونهای الایزا، RT-PCR و RT-qPCR در تیمار ترکیبی کشت مریستم با اندازه 3/0 میلیمتر و گرمادرمانی 50 درجه سلسیوس به مدت 60 دقیقه، به میزان 100 درصد بدست آمد. این مطالعه، یک روش موثر برای توسعه بنههای زعفران بدون ویروس ارائه میکند، به طوریکه میتواند برای تکثیر گیاه عاری از ویروس و پایداری صنعت تولید زعفران، نقش مهمی ایفا نماید. | ||
| کلیدواژهها | ||
| الایزا؛ زعفران؛ ویروس زدایی؛ RT-PCR؛ RT-qPCR | ||
| مراجع | ||
منابعشکرپور، مجید، عابدی، زینب، کلانتری، سیامک و سلامی، سید علیرضا. (1395). بررسی تنوع ژنتیکی برخی نمونههای زعفران ایران با استفاده از نشانگرهای ملکولی RAPD و ISSR. زراعت و فناوری زعفران، 4 (4)، 257-267. https://doi.org/10.22048/jsat.2016.38672 REFERENCES Ágoston, J., Almási, A., Pinczés, D., Sáray, R., Salánki, K., & Palkovics, L. (2024). First report of saffron latent virus in Crocus sativus from Hungary. Plant Disease, 108(2), 540. https://doi.org/10.1094/PDIS-09-23-1765-PDN Ahrazem, O., Argandoña, J., Castillo, R., Rubio-Moraga, A., & Gómez-Gómez, L. (2016). Identification and cloning of differentially expressed SOUL and ELIP genes in saffron stigmas using a subtractive hybridization approach. PloS One, 11(12), e0168736. https://doi.org/10.1371/journal.pone. 0168736 Bayati, S., Shams-Bakhsh, M., & Moini, A. (2011). Elimination of Grapevine virus A (GVA) by cryotherapy and electrotherapy. Journal of Agricultural Science and Technology, 13(3), 442-450. In Persian. https://doi.org/20.1001.1.16807073.2011.13.3.2.9 Benke, A. P., Krishna, R., Khandagale, K., Gawande, S., Shelke, P., Dukare, S., ... & Mahajan, V. (2023). Efficient elimination of viruses from garlic using a combination of shoot meristem culture, thermotherapy, and chemical treatment. Pathogens, 12(1), 129. https://doi.org/10.3390/ pathogens12010129 Bruni, R., Bellardi, M. G., & Parrella, G. (2016). Change in chemical composition of sweet basil (Ocimum basilicum L.) essential oil caused by alfalfa mosaic virus. Journal of Phytopathology, 164(3), 202-206. https://doi.org/10.1111/jph.12410 Caiola, M. G., & Faoro, F. (2011). Latent virus infections in Crocus sativus and Crocus cartwrightianus. Phytopathologia Mediterranea, 50(2), 175-182. Cervera, H., Ambrós, S., Bernet, G. P., Rodrigo, G., & Elena, S. F. (2018). Viral fitness correlates with the magnitude and direction of the perturbation induced in the hostˈs transcriptome: the tobacco etch potyvirus—tobacco case study. Molecular Biology and Evolution, 35(7), 1599-1615. https://doi.org/10. 1093/ molbev/msy038 Chakraborty, S. (2016). Transcriptome from saffron (Crocus sativus) plants in Jammu and Kashmir reveals abundant soybean mosaic virus transcripts and several putative pathogen bacterial and fungal genera. BioRxiv, 079186. https://doi.org/10.1101/079186 Chen, J. (2000). Occurrence and control of mosaic disease [turnip mosaic virus] in saffron (Crocus sativus). Zhejiang Nongye Kexue, 3, 132-135. Gogile, A., Markos, T., Kebede, M., Kidanemariam, D., & Abraham, A. (2024). Elimination of yam mosaic virus from yam using an optimized combination of meristem culture and thermotherapy. Australasian Plant Pathology, 53(2), 185-197. https://doi.org/10.1007/s13313-024-00965-9 González‐Jara, P., Tenllado, F., Martínez‐García, B., Atencio, F. A., Barajas, D., Vargas, M., ... & Díaz‐Ruíz, J. R. (2004). Host‐dependent differences during synergistic infection by Potyviruses with potato virus X. Molecular Plant Pathology, 5(1), 29-35. https://doi.org/10.1111/j.1364-3703.2004.00202.x Haseli M., Valouzi H. & Dizadji A. (2024). Beet western yellows virus frequently infects Crocus sativus in Iran. Australasian Plant Disease Notes, 19(1). https://doi.org/10.1007/s13314-024-00538-1 Hu, G., Dong, Y., Zhang, Z., Fan, X., Ren, F., & Zhou, J. (2015). Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Plant Cell, Tissue and Organ Culture (PCTOC), 121, 435-443. https://doi.org/10.1007/s11240-015-0714-6 Kazemi, N., Habashi, A. A., & Asadi, W. (2019). Evaluation of combined treatments of thermotherapy and apical meristem culture efficiencyon virus elimination from in vitro shootlts of Red Flash Apple (Malus pumila Mill.). Journal of Horticultural Science. 33(3), 499-509. https://doi /epdf/10.5555/ 20203567082 Kim, Y., Kim, Y. J., & Paek, K. H. (2021). Temperature-specific vsiRNA confers RNAi-mediated viral resistance at elevated temperature in Capsicum annuum. Journal of Experimental Botany, 72(4), 1432-1448. https://doi.org/10.1093/jxb/eraa527 Křižan, B., Ondrušiková, E., Holleinová, V., Moravcova, K., & Blahova, L. (2009). Elimination of Grapevine fanleaf virus in grapevine by in vivo and in vitro thermotherapy. Horticultural Science, 36(3), 105-108. https://doi.org/10.17221/37/2008-HORTSCI Kwon, Y. H., Choi, W. I., Kim, H. K., Kim, K. O., Kim, J. H., Huh, Y. S., & Park, W. T. (2022). Efficacy of virus elimination from 'Rehmannia glutinosa' using simultaneous thermotherapy, chemotherapy, and meristem culture. Plant Omics, 15(1), 6-12. https://doi.org/10.21475/ POJ.15.01.22.p3527 Leone, S., Recinella, L., Chiavaroli, A., Orlando, G., Ferrante, C., Leporini, L., & Menghini, L. (2018). Phytotherapic use of the Crocus sativus L. (Saffron) and its potential applications: A brief overview. Phytotherapy Research, 32(12), 2364-2375. https://doi.org/10.1002/ptr.6181 Liu, J., Zhang, X., Yang, Y., Hong, N., Wang, G., Wang, A., & Wang, L. (2016). Characterization of virus-derived small interfering RNAs in Apple stem grooving virus-infected in vitro-cultured Pyrus pyrifolia shoot tips in response to high temperature treatment. Virology Journal, 13, 1-11. https://doi.org/10.1186/s12985-016-0625-0 Lorenzo, C., Shadmani, G., Valouzi, H., Moratalla-López, N., Bahlolzada, H., Sánchez-Gómez, R., ... & Alonso, G. L. (2023). Saffron Stigmas Apocarotenoid Contents from Saffron Latent Virus (SaLV)-Infected Plants with Different Origins and Dehydration Temperatures. Horticulturae, 9(8), 933. https://doi.org/10.3390/horticulturae9080933 Martinez-Fajardo, C., Navarro-Simarro, P., Morote, L., Rubio-Moraga, Á., Mondéjar-López, M., Niza, E., ... & López-Jiménez, A. J. (2024). Exploring the viral landscape of saffron through metatranscriptomic analysis. Virus Research, 345, 199389. https://doi.org/10.1016/ j.virusres.2024.199389 Miglino, R., Jodlowska, A., & Van Schadewijk, A. R. (2005). First report of Narcissus mosaic virus infecting Crocus spp. cultivars in the Netherlands. Plant Disease, 89(3), 342-342. https://doi.org/10.1094/PD-89-0342C Miljanić, V., Rusjan, D., Škvarč, A., Chatelet, P., & Štajner, N. (2022). Elimination of eight viruses and two viroids from preclonal candidates of six grapevine varieties (Vitis vinifera L.) through in vivo thermotherapy and in vitro meristem tip micrografting. Plants, 11(8), 1064. https://doi.org/10.3390/plants11081064 Moratalla-López, N., Parizad, S., Habibi, M. K., Winter, S., Kalantari, S., Bera, S., ... & Alonso, G. L. (2021). Impact of two different dehydration methods on saffron quality, concerning the prevalence of Saffron latent virus (SaLV) in Iran. Food Chemistry, 337, 127786. https://doi.org/10.1016/j.foodchem.2020.127786 Movi, S., Dizadji, A., Parizad, S., & Zarghani, S. N. (2022). Biological characteristics and genetic variation analyses of saffron latent virus (SaLV) based on genomic P1-Pro and P3 regions. European Journal of Plant Pathology, 164(2), 299-312. https://doi.org/10.1007/s10658-022-02561-3 Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473—497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x Nesi, B., Trinchello, D., Lazzereschi, S., Grassotti, A., & Ruffoni, B. (2009). Production of lily symptomless virus-free plants by shoot meristem tip culture and in vitro thermotherapy. HortScience, 44(1), 217-219. https://doi.org/10.21273/HORTSCI.44.1.217 Panattoni, A., Luvisi, A., & Triolo, E. (2013). Elimination of viruses in plants: twenty years of progress. Spanish Journal of Agricultural Research, 11(1), 173-188. http://dx.doi.org/10.5424/sjar/2013111-3201 Parizad, S., Dizadji, A., Habibi, M. K., Winter, S., Kalantari, S., Movi, S., ... & Moratalla-Lopez, N. (2019). The effects of geographical origin and virus infection on the saffron (Crocus sativus L.) quality. Food Chemistry, 295, 387-394. https://doi.org/10.1016/j.foodchem.2019.05.116 Parizad, S., Dizadji, A., Koohi Habibi, M., Winter, S., Kalantari, S., Movi, S., ... & Ayllón, M. A. (2018). Description and genetic variation of a distinct species of potyvirus infecting saffron (Crocus sativus L.) plants in major production regions in Iran. Annals of Applied Biology, 173(3), 233-242. https://doi.org/10.1111/aab.12456 Petrov, N. M., Stoyanova, M. I., & Gaur, R. K. (2024). Characterization and management of economically important viruses on sweet pepper cultivars in Europe. In Pepper Virome (pp. 445-475). Academic Press. https://doi.org/10.1016/B978-0-443-15576-5.00010-1 Ramírez-Malagón, R., Pérez-Moreno, L., Borodanenko, A., Salinas-González, G. J., & Ochoa-Alejo, N. (2006). Differential organ infection studies, potyvirus elimination, and field performance of virus-free garlic plants produced by tissue culture. Plant Cell, Tissue and Organ Culture, 86, 103-110. https://doi.org/10.1007/s11240-006-9102-6 Shabala, S. (2012). Plant stress physiology, CABi Publishing, (Shabala, S., & Bose, J. 2012: 91–126). Shalitin, D., & Wolf, S. (2000). Cucumber mosaic virus infection affects sugar transport in melon plants. Plant Physiology, 123(2), 597-604. https://doi.org/10.1104/pp.123.2.597 Sharifi Nezamabad, P., Koohi Habibi, M., Dizadji, A., & Kalantari, S. (2015). Elimination of Bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms. Journal of Crop Protection, 4(4), 533-543. https://doi.org/20.1001.1.22519041.2015.4.4.10.8 Shokrpour, M. (2019). Saffron (Crocus sativus L.) breeding: opportunities and challenges. In Advances in Plant Breeding Strategies: Industrial and Food Crops, edited by Al-Khayri, J., Jain, S., Johnson, D.. Springer, Cham. : Volume 6, 675-706. https://doi.org/10.1007/978-3-030-23265-8_17 Tan, R., Wang, L., Hong, N., & Wang, G. (2010). Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear. Plant Cell, Tissue and Organ Culture (PCTOC), 101, 229-235. https://doi.org/10.1007/s11240-010-9681-0 Valouzi, H., Dizadji, A., Golnaraghi, A., Salami, S.A., Selmi, I., Fontdevila Pareta, N. et al. (2025) First detection of saffron dwarf virus, wheat dwarf virus, wheat dwarf virus-associated alphasatellite and a new putative potyvirus species in saffron in Iran. New Disease Reports, vol.51, e70022. https://doi.org/10.1002/ndr2.70022 Vieira, R. L., da Silva, A. L., Zaffari, G. R., Steinmacher, D. A., de Freitas Fraga, H. P., & Guerra, M. P. (2015). Efficient elimination of virus complex from garlic (Allium sativum L.) by cryotherapy of shoot tips. Acta Physiologiae Plantarum, 37, 1-11. https://doi.org/10.1007/s11738-014-1733-3 Vivek, M., & Modgil, M. (2018). Elimination of viruses through thermotherapy and meristem culture in apple cultivar ‘Oregon Spur-II’. Virus Disease, 29, 75-82. https://doi.org/10.1007/s13337-018-0437-5 Wang, L., Wang, G., Hong, N., Tang, R., Deng, X., & Zhang, H. (2006). Effect of thermotherapy on elimination of Apple stem grooving virus and Apple chlorotic leaf spot virus for in vitro-cultured pear shoot tips. HortScience, 41(3), 729-732. https://doi.org/10.21273/HORTSCI.41.3.729 Wang, M. R., Cui, Z. H., Li, J. W., Hao, X. Y., Zhao, L., & Wang, Q. C. (2018). In vitro thermotherapy-based methods for plant virus eradication. Plant methods, 14, 1-18. https://doi.org/10.1186/s13007-018-0355-y Wang, M. R., Hamborg, Z., Blystad, D. R., & Wang, Q. C. (2021). Combining thermotherapy with meristem culture for improved eradication of onion yellow dwarf virus and shallot latent virus from infected in vitro‐cultured shallot shoots. Annals of Applied Biology, 178(3), 442-449. https://doi.org/10.1111/aab.12646 Wang, Q., Cuellar, W. J., Rajamaki, M. L., Hirata, Y., & Valkonen, J. P. (2008). Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology, 9(2), 237-250. https://doi.org/10.1111/j.1364-3703.2007.00456.x Zheng, H. Y., Wu, X. Y., Han, K. L., Chen, Z. Q., Song, X. J., Peng, J. J., ... & Han, K. L. (2018). First Report of Beet western yellows virus Infecting Crocus sativus in China. Plant Disease, 102(7), 1471-1471. https://doi.org/10.1094/PDIS-10-17-1579-PDN | ||
|
آمار تعداد مشاهده مقاله: 554 تعداد دریافت فایل اصل مقاله: 128 |
||