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akhlaghi, parisa, Mehdinavaz Aghdam, Rouhollah, Ashouri Sharafshadeh, Sina, Pirhadi Tavandashti, Yalda. (1404). Fabrication of Amniotic Membrane coated Silk Fibroin nanofibers Containing Thymol for Antibacterial Wound Dressing. , 58(1), 1-14. doi: 10.22059/jufgnsm.2025.01.01
parisa akhlaghi; Rouhollah Mehdinavaz Aghdam; Sina Ashouri Sharafshadeh; Yalda Pirhadi Tavandashti. "Fabrication of Amniotic Membrane coated Silk Fibroin nanofibers Containing Thymol for Antibacterial Wound Dressing". , 58, 1, 1404, 1-14. doi: 10.22059/jufgnsm.2025.01.01
akhlaghi, parisa, Mehdinavaz Aghdam, Rouhollah, Ashouri Sharafshadeh, Sina, Pirhadi Tavandashti, Yalda. (1404). 'Fabrication of Amniotic Membrane coated Silk Fibroin nanofibers Containing Thymol for Antibacterial Wound Dressing', , 58(1), pp. 1-14. doi: 10.22059/jufgnsm.2025.01.01
akhlaghi, parisa, Mehdinavaz Aghdam, Rouhollah, Ashouri Sharafshadeh, Sina, Pirhadi Tavandashti, Yalda. Fabrication of Amniotic Membrane coated Silk Fibroin nanofibers Containing Thymol for Antibacterial Wound Dressing. , 1404; 58(1): 1-14. doi: 10.22059/jufgnsm.2025.01.01

Fabrication of Amniotic Membrane coated Silk Fibroin nanofibers Containing Thymol for Antibacterial Wound Dressing

Journal of Ultrafine Grained and Nanostructured Materials
دوره 58، شماره 1، شهریور 2025، صفحه 1-14    اصل مقاله (1.15 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22059/jufgnsm.2025.01.01
نویسندگان
parisa akhlaghi1؛ Rouhollah Mehdinavaz Aghdam* 1؛ Sina Ashouri Sharafshadeh1؛ Yalda Pirhadi Tavandashti2
1Biomaterials Lab, School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran.
2Biomaterials Lab, School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran
چکیده
Bacterial wound infection delays wound healing and may lead to life-threatening complications. Hence wound dressing materials are not restricted to stop blood loss, but also to protect the wound from bacterial infection and to accelerate the wound healing process. In this study, biopolymeric nanofibers of Silk Fibroin (SF) and decellularized human Amniotic membrane (dehAM) were fabricated by electrospinning technique to be used in wound healing. Thymol, a natural antibacterial substance, solutions containing 5% wt/wt, 10%wt/wt and 15% wt/wt thymol was added to the SF nanofibers to increase the sustained release. Scanning Electron Microscopy and Attenuated Total Reflectance Fourier-Transform Infrared (FTIR-ATR) were used to examine the nanofibers' morphology and chemical characteristics. Water contact angle and water vapor transmission rate of dressings were measured. When the thymol concentration of the dressing was increased, the wettability of the dressing decreased by an average of 54%. The dressings' antimicrobial activity was determined using the colony count method, which dressings effectively inhibited the development of E. coli and S. aureus. Furthermore, cell attachment, cell viability, and in vitro cytotoxicity experiments have demonstrated the biocompatibility and cytocompatibility of the dressings, as well as their suitability for MSCs attachment. Altogether, the produced dressing was shown to have immense potential as a wound dressing.
کلیدواژه‌ها
Wound dressing؛ Electrospinning؛ Thymol؛ Human Amniotic Membrane, Antibacterial agent
مراجع
 

  1. M. F. P. Graça, S. P. Miguel, C. S. D. Cabral, and I. J. Correia, “Hyaluronic acid—Based wound dressings: A review,” Carbohydr. Polym., vol. 241, 2020.
  2. D. Simões, S. P. Miguel, M. P. Ribeiro, P. Coutinho, A. G. Mendonça, and I. J. Correia, “Recent advances on antimicrobial wound dressing: A review,” Eur. J. Pharm. Biopharm., vol. 127, no. December 2017, pp. 130–141, 2018.
  3. R. Ambekar, B. K.-E. P. Journal, and undefined 2019, “Advancements in nanofibers for wound dressing: A review,” Elsevier.
  4. A. Gupta, M. Kowalczuk, W. Heaselgrave, S. T. Britland, C. Martin, and I. Radecka, “The production and application of hydrogels for wound management: A review,” Eur. Polym. J., vol. 111, no. November 2018, pp. 134–151, 2019.
  5. G. Han and R. Ceilley, “Chronic Wound Healing: A Review of Current Management and Treatments,” Adv. Ther., vol. 34, no. 3, pp. 599–610, Mar. 2017.
  6. S. Homaeigohar and A. R. Boccaccini, “Antibacterial biohybrid nanofibers for wound dressings,” Acta Biomater., vol. 107, no. 2020, pp. 25–49, 2020.
  7. I. Negut, V. Grumezescu, and A. M. Grumezescu, “Treatment strategies for infected wounds,” Molecules, vol. 23, no. 9, pp. 1–23, 2018.
  8. A. Davis, K. B.-D. S. Journal, and undefined 2016, “Bioactive Hybrid Composite Membrane with Enhanced Antimicrobial Properties for Biomedical Applications.,” search.ebscohost.com.
  9. G. Sun, X. Zhang, Y. Shen, … R. S.-P. of the, and undefined 2011, “Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing,” Natl. Acad Sci.
  10. D. Altiok, E. Altiok, F. T.-J. of M. S. Materials, and undefined 2010, “Physical, antibacterial and antioxidant properties of chitosan films incorporated with thyme oil for potential wound healing applications,” Springer, vol. 21, no. 7, pp. 2227–2236, Jul. 2010.
  11. S. Anjum, A. Sharma, M. Tummalapalli, J. Joy, S. Bhan, and B. Gupta, “A novel route for the preparation of silver loaded polyvinyl alcohol nanogels for wound care systems,” Int. J. Polym. Mater. Polym. Biomater., vol. 64, no. 17, pp. 894–905, Dec. 2015.
  12. S. Kumar et al., “Development of novel chitin/nanosilver composite scaffolds for wound dressing applications,” Springer, vol. 21, no. 2, pp. 807–813, Feb. 2009.
  13. A. E. Purushothaman, K. Thakur, and B. Kandasubramanian, “Development of highly porous, Electrostatic force assisted nanofiber fabrication for biological applications,” Int. J. Polym. Mater. Polym. Biomater., vol. 69, no. 8, pp. 477–504, May 2020.
  14. R. Yadav, K. B.-E. of Nanobiomaterials, and undefined 2016, “Bioabsorbable engineered nanobiomaterials for antibacterial therapy,” Elsevier.
  15. V. Andreu, G. Mendoza, M. Arruebo, and S. Irusta, “Smart Dressings Based on Nanostructured Fibers Containing Natural Origin Antimicrobial, Anti-Inflammatory, and Regenerative Compounds,” Mater. 2015, Vol. 8, Pages 5154-5193, vol. 8, no. 8, pp. 5154–5193, Aug. 2015.
  16. P. Zahedi, I. Rezaeian, S. O. Ranaei-Siadat, S. H. Jafari, and P. Supaphol, “A review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages,” Polym. Adv. Technol., vol. 21, no. 2, pp. 77–95, Feb. 2010.
  17. Z. Liu, S. Shang, K. lok Chiu, S. Jiang, and F. Dai, “Fabrication of silk fibroin/poly(lactic-co-glycolic acid)/graphene oxide microfiber mat via electrospinning for protective fabric,” Mater. Sci. Eng. C, vol. 107, no. October 2019, p. 110308, 2020.
  18. P. P. Patil, M. R. Reagan, and R. A. Bohara, “Silk fibroin and silk-based biomaterial derivatives for ideal wound dressings,” Int. J. Biol. Macromol., 2020.
  19. M. Pollini, “Bioinspired Materials for Wound Healing Application : The Potential of Silk Fibroin,” 2020.
  20. M. Farokhi, F. Mottaghitalab, Y. Fatahi, A. Khademhosseini, and D. L. Kaplan, “Overview of Silk Fibroin Use in Wound Dressings,” Trends Biotechnol., vol. 36, no. 9, pp. 907–922, 2018.
  21. D. Chouhan and B. B. Mandal, “Silk Biomaterials in Wound Healing and Skin Regeneration Therapeutics: from Bench to Bedside,” Acta Biomater., 2019.
  22. M. Ghanbari, R. Karimian, and B. Mehramouz, “International Journal of Biological Macromolecules Preparation of biocompatible and biodegradable silk fi broin / chitin / silver nanoparticles 3D scaffolds as a bandage for antimicrobial wound dressing,” vol. 114, pp. 961–971, 2018.
  23. A. Rodella, M. Pozzobon, M. Rigon, C. Franchin, and G. Arrigoni, “Topical application of lyophilized and powdered human amniotic membrane promotes diabetic ulcer healing,” Wound Med., vol. 27, no. 1, p. 100171, 2019.
  24. A. Rezaei, A. Ali, M. Amir, and M. Mollapour, “Update review on five top clinical applications of human amniotic membrane in regenerative medicine,” Placenta, vol. 103, no. September 2020, pp. 104–119, 2021.
  25. S. V. Murphy et al., “Amnion membrane hydrogel and amnion membrane powder accelerate wound healing in a full thickness porcine skin wound model,” Stem Cells Transl. Med., vol. 9, no. 1, pp. 80–92, Jan. 2020.
  26. L. Sara et al., “Human Amniotic Membrane : A review on tissue engineering , application , and storage,” no. November, pp. 1–18, 2020.
  27. A. Marchese et al., “Antibacterial and antifungal activities of thymol: a brief review of the literature,” FOOD Chem., 2016.
  28. S. P. Miguel, D. Simões, A. F. Moreira, S. Rosa, and I. J. Correia, “Production and characterization of electrospun silk fibroin based asymmetric membranes for wound dressing applications,” Int. J. Biol. Macromol., 2018.
  29. R. Najafloo, M. Behyari, R. Imani, and S. Nour, “A Mini-review of Thymol Incorporated Materials: Applications in Antibacterial Wound Dressing,” J. Drug Deliv. Sci. Technol., p. 101904, 2020.
  30. Y. Chen, Y. Qiu, W. Chen, and Q. Wei, “Materials Science & Engineering C Electrospun thymol-loaded porous cellulose acetate fi bers with potential biomedical applications,” Mater. Sci. Eng. C, vol. 109, no. December 2019, p. 110536, 2020.
  31. G. Hong, J., Yeo, M., Yang, G. H., & Kim, “Cell-electrospinning and its application for tissue engineering,” Int. J. Mol. Sci., vol. 20, 2019.
  32. H. Yang, X., Fan, L., Ma, L., Wang, Y., Lin, S., Yu, F., ... & Wang, “Green electrospun Manuka honey/silk fibroin fibrous matrices as potential wound dressing,” Mater. Des., vol. 119, pp. 76–84, 2017.
  33. A. Basal, G., Tetik, G. D., Kurkcu, G., BAYRAKTAR, O., Gurhan, I. D., & Atabey, “olive leaf extract loaded silk fibroin/hyaluronic acid nanofiber webs for wound dressing applications,” Dig. J. Nanomater. Biostructures, vol. 11, 2016.
  34. B. Kheradvar, S. A., Nourmohammadi, J., Tabesh, H., & Bagheri, “Starch nanoparticle as a vitamin E-TPGS carrier loaded in silk fibroin-poly (vinyl alcohol)-Aloe vera nanofibrous dressing,” Colloids Surfaces B Biointerfaces, vol. 166, pp. 9–16, 2018.
  35. D. Rockwood, R. Preda, T. Yücel, X. W.-N. protocols, and undefined 2011, “Materials fabrication from Bombyx mori silk fibroin,” nature.com.
  36. R. H. Pan, Z., Bora, M., Gee, R., & Dauskardt, “Water vapor transmission rate measurement for moisture barriers using infrared imaging,” Mater. Chem. Phys., vol. 308, 2023.
  37. G. Sen, S., Das, C., Ghosh, N. N., Baildya, N., Bhattacharya, S., Khan, M. A., ... & Biswas, “Is degradation of dyes even possible without using photocatalysts?–a detailed comparative study,” RSC Adv., vol. 12, 2022.
  38. M. Zafari et al., “Physical and biological properties of blend-electrospun polycaprolactone/chitosan-based wound dressings loaded with N-decyl-N, N-dimethyl-1-decanaminium chloride: An in vitro and in vivo study,” J. Biomed. Mater. Res. - Part B Appl. Biomater., vol. 108, no. 8, pp. 3084–3098, 2020.
  39. X. Yu, B., He, C., Wang, W., Ren, Y., Yang, J., Guo, S., ... & Shi, “Asymmetric wettable composite wound dressing prepared by electrospinning with bioinspired micropatterning enhances diabetic wound healing,” ACS Appl. Bio Mater., vol. 3, 2020.
  40. D. Yang, Z. Fang, R. Kang, K. L.-M. & Design, and undefined 2021, “Composite electrospun scaffold containing decellularized amniotic matrix for pelvic organ prolapse,” Elsevier.
  41. A. Ghaee, M. Karimi, … M. L.-S.-M. S. and, and undefined 2019, “Preparation of hydrophilic polycaprolactone/modified ZIF-8 nanofibers as a wound dressing using hydrophilic surface modifying macromolecules,” Elsevier.
  42. M. Y. Hajibonabi, A., Yekani, M., Sharifi, S., Nahad, J. S., Dizaj, S. M., & Memar, “Antimicrobial activity of nanoformulations of carvacrol and thymol: New trend and applications,” OpenNano, vol. 13, 2023.
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