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Jelodar, Abbas, Chehari, Kobra, Jafari, Hediyeh. (1404). Genetic Variability of Hemiscorpius lepturus in Khuzestan using ISSR-PCR and Mitochondrial Cytochrome C Oxidase Subunit I Gene sequencing. , (), -. doi: 10.22059/ijvm.2025.388996.1005726
Abbas Jelodar; Kobra Chehari; Hediyeh Jafari. "Genetic Variability of Hemiscorpius lepturus in Khuzestan using ISSR-PCR and Mitochondrial Cytochrome C Oxidase Subunit I Gene sequencing". , , , 1404, -. doi: 10.22059/ijvm.2025.388996.1005726
Jelodar, Abbas, Chehari, Kobra, Jafari, Hediyeh. (1404). 'Genetic Variability of Hemiscorpius lepturus in Khuzestan using ISSR-PCR and Mitochondrial Cytochrome C Oxidase Subunit I Gene sequencing', , (), pp. -. doi: 10.22059/ijvm.2025.388996.1005726
Jelodar, Abbas, Chehari, Kobra, Jafari, Hediyeh. Genetic Variability of Hemiscorpius lepturus in Khuzestan using ISSR-PCR and Mitochondrial Cytochrome C Oxidase Subunit I Gene sequencing. , 1404; (): -. doi: 10.22059/ijvm.2025.388996.1005726

Genetic Variability of Hemiscorpius lepturus in Khuzestan using ISSR-PCR and Mitochondrial Cytochrome C Oxidase Subunit I Gene sequencing

Iranian Journal of Veterinary Medicine
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 04 خرداد 1404
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22059/ijvm.2025.388996.1005726
نویسندگان
Abbas Jelodar* 1؛ Kobra Chehari2؛ Hediyeh Jafari3
1Department of Basic Sciences, Biochemistry and Molecular Biology Section, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, 61355, P.O.Box 145 Ahvaz, Iran
2PhD student in the Faculty of Veterinary Medicine at the Shahid Chamran University of Ahvaz, Ahvaz, Iran; 2Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3Razi Vaccines and Serum Research Institute, Agricultural Research education and extension Organization, Ahvaz, Iran.
چکیده
Background: Scorpion stings pose a serious public health concern, particularly in Khuzestan Province, Iran. The genus Hemiscorpius scorpions are a major cause to scorpion sting and its related death mostly in children.
Objectives: we have used Inter simple sequence repeat anchored polymerase chain reaction (ISSR-PCR) and cytochrome c oxidase subunit 1 (COI) to study potential intraspecies variability of this scorpion.
Methods: Twenty-two specimens of Hemiscorpius lepturus scorpion were collected from 5 geographically different regions of Khuzestan regions. Genomic DNA extraction was performed using phenol / chloroform method. For phylogeny analysis, target gene fragments were amplified by ISSR-PCR technique. By agarose gel electrophoresis of the PCR products, bands produced in each specimen were categorized using zero and one system and dendrogram was drawn using the UPGMA algorithm.
Results: ISSR-PCR generated 5 bands ranged from 0.9-2.5 kb. The results showed that specimen Hl4Ch was clearly different within their groups. To validate these findings, a 637-nucleotide fragment of the COI gene was amplified and sequenced from five genetically variable specimens. Out of 5 sequences, Hl1Ba and Hl6Be have a relatively close relationship (57%) with H. lepturus reference sequence (KU341987). However, Hl4Ch was placed with a relatively high distance (72%) from the rest of them, next to the other reference sequence Hemiscorpius sp. (OP433762.1).
Conclusion: Although the scorpions of each region were mostly placed together in the phylogeny tree, no major genetic diversity related to regional differences was observed in the province. Based on the genetic distance of Hl4Ch from other sequences (12.5%), it can be concluded that it is definitely an intraspecies variation.
کلیدواژه‌ها
dendrogram؛ Inter simple sequence repeat؛ Khuzestan؛ Phylogeny؛ Scorpion
آمار
تعداد مشاهده مقاله: 28





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