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Vazifehdoust, Ghazale, Jamshidi, Shahram, Nikbakht, Gholamreza, Taheri, Mohammad, lalepoor, Mahya. (1404). Characterization of Serum Concentration CD8, CD4 ,IFN-γ and IL-4 in 4T1 Tumor Inoculated Mouse Model. , (), -. doi: 10.22059/ijvm.2025.378508.1005605
Ghazale Vazifehdoust; Shahram Jamshidi; Gholamreza Nikbakht; Mohammad Taheri; Mahya lalepoor. "Characterization of Serum Concentration CD8, CD4 ,IFN-γ and IL-4 in 4T1 Tumor Inoculated Mouse Model". , , , 1404, -. doi: 10.22059/ijvm.2025.378508.1005605
Vazifehdoust, Ghazale, Jamshidi, Shahram, Nikbakht, Gholamreza, Taheri, Mohammad, lalepoor, Mahya. (1404). 'Characterization of Serum Concentration CD8, CD4 ,IFN-γ and IL-4 in 4T1 Tumor Inoculated Mouse Model', , (), pp. -. doi: 10.22059/ijvm.2025.378508.1005605
Vazifehdoust, Ghazale, Jamshidi, Shahram, Nikbakht, Gholamreza, Taheri, Mohammad, lalepoor, Mahya. Characterization of Serum Concentration CD8, CD4 ,IFN-γ and IL-4 in 4T1 Tumor Inoculated Mouse Model. , 1404; (): -. doi: 10.22059/ijvm.2025.378508.1005605

Characterization of Serum Concentration CD8, CD4 ,IFN-γ and IL-4 in 4T1 Tumor Inoculated Mouse Model

Iranian Journal of Veterinary Medicine
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 15 اردیبهشت 1404
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22059/ijvm.2025.378508.1005605
نویسندگان
Ghazale Vazifehdoust* 1؛ Shahram Jamshidi1؛ Gholamreza Nikbakht2؛ Mohammad Taheri3؛ Mahya lalepoor4
1Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3Dr. Rastegar Laboratories, Faculty of veterinary medicine, University of Tehran, Tehran, Iran.
4Department of pathology, university of Tehran, Tehran, Iran
چکیده
Malignancy poses a significant health risk across various species, particularly in domesticated animals such as cats and dogs. Despite initial tumor therapy screening efforts using murine models to elucidate tumor biology, the development and validation of novel therapeutic strategies necessitate the use of more sophisticated models. This study aims to characterize the immune system alterations in a 4T1 tumor-inoculated mouse model by assessing serum concentrations of CD4 and CD8 lymphocytes and cytokines (IL-4, IFN-γ). 4T1 cells were cultured and inoculated subcutaneously into BALB/c female mice. Serum concentrations of CD4 and CD8 lymphocytes, as well as cytokines IL-4 and IFN-γ, were measured using ELISA. Histopathological analysis of the tumors was conducted to evaluate the immunosuppressive environment. CD8 levels were significantly higher in tumor-inoculated mice compared to healthy controls (mean: 7.2613 ng/ml vs. 1.9749 ng/ml). Conversely, CD4 levels were significantly lower in the tumor group (mean: 2.7632 ng/ml vs. 4.8677 ng/ml). IFN-γ levels were reduced in the tumor-inoculated group, indicating an immunosuppressive environment (mean: 10.95238 pg/ml vs. 22.85714 pg/ml). IL-4 levels were significantly elevated in the tumor group,(mean: 27.76942 pg/ml vs. 10.85452 pg/ml). The study highlights significant immune system alterations in the 4T1 tumor model, characterized by increased CD8 levels, reduced CD4 and IFN-γ levels, and elevated IL-4 levels. These findings highlights the importance of understanding the immune-tumor interactions to address cancer immune evasion and design effective immunotherapies. The 4T1 model provides a valuable platform for future immunotherapy research, offering insights into the immune responses and potential therapeutic strategies in a clinically relevant context.
کلیدواژه‌ها
animals؛ cancer؛ cytokines؛ immune response؛ mouse model
آمار
تعداد مشاهده مقاله: 17





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