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مطالعة اثر سمیت ذرات احیاء شدة نقره کلوئیدی با استفاده از عصارة الکلی بره موم پرتوتابی شده و نمک نیترات نقره بر ریز جلبک Chlorella vulgaris | ||
| شیلات | ||
| دوره 76، شماره 3، مهر 1402، صفحه 423-435 اصل مقاله (638.9 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jfisheries.2023.355590.1371 | ||
| نویسندگان | ||
| سیدمحمودرضا آقامیری1؛ مرضیه حیدریه* 2؛ سعیده نعیمی3؛ آوا رضایی4 | ||
| 1استاد، گروه آموزشی پرتوپزشکی، دانشکده مهندسی هسته‌ای، دانشگاه شهید بهشتی، تهران، ایران | ||
| 2دانشیار، پژوهشگاه علوم و فنون هسته ای، تهران، ایران | ||
| 3استادیار، گروه آموزشی علومپایه، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران | ||
| 4دکتری دامپزشکی، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران | ||
| چکیده | ||
| انتشار نانوذرات، از جمله نانوذرات نقره استفاده شده در صنایع مختلف به محیط زیست و اثرات سمی احتمالی آنها بر رشد گیاهان و سلامت انسانها نگرانی جامعه را فراهم آورده و نیاز به تحقیق و بررسی در این زمینه را پررنگتر نموده است. هدف از این مطالعه بررسی اثر سمیت مواجهه با غلظتهای مختلف (10، 50، 100 و 200 میکروگرم/لیتر) ذرات احیاء شده نقره با استفاده از عصارة الکلی برهموم پرتودهی شده با پرتو گاما در سطح 10 کیلوگری (AgNPs-γ)، ذرات احیاء شدة نقره با استفاده از عصارة الکلی بره موم پرتودهی نشده (AgNPs) و نمک نیترات نقره (AgNO3) بر ریزجلبکهای کلرلا ولگاریس (Chlorella vulgaris) بود. نتایج نشان داد که درصد مهار رشد در گروههای مواجهه یافته با AgNPs-γ و AgNPs و AgNO3 به‌صورت AgNo3 وابسته به غلظت افزایش یافت (0/05>P). همچنین در این گروهها درصد زندهمانی سلولها، غلظت جلبک (میکرو/لیتر) و میزان کلروفیل a کاهش و درصد تغییر شاخص هدایت الکترولیتی/سلول زنده (میزان نشت الکترولیتی) به‌صورت معنی داری افزایش یافت (0/05>P). براساس نتایج به‌دست آمده، ببه‌ترتیب AgNo3<AgNPs< AgNPs-γ موجب ایجاد سمیت و کاهش رشد و فتوسنتز در ریزجلبک کلرلا ولگاریس میشوند. | ||
| کلیدواژه‌ها | ||
| بره موم؛ ذرات احیاء شده نقره؛ پرتوتابی؛ کلرلا ولگاریس | ||
| عنوان مقاله [English] | ||
| Toxic Effects of Colloidal Silver Particles Biosynthesized using Gamma-irradiated Propolis Extract and Silver Nitrate Salts on Microalgae Chlorella vulgaris | ||
| نویسندگان [English] | ||
| Seyed Mahmoud Reza Aghamiri1؛ Marzieh Heidarieh2؛ Saeideh Naeimi3؛ Ava Resae4 | ||
| 1Professor, Medical Radiation Engineering, Shahid Beheshti University, Tehran, Iran | ||
| 2Associate professor, Nuclear Science and Technology Research Institute, Tehran, Iran | ||
| 3Assistant Professor in the Department of Basic Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| 4DVM, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| چکیده [English] | ||
| The discharge of nanoparticles, including silver nanoparticles used in various industries into the environment, and their potential toxic effects on plants, animals and humans has raised global concerns, highlighting the need for more research on this topic. The aim of this study was to investigate the toxicity of silver nitrate salt (AgNO3), γ-irradiated (10 kGy) (AgNPs-γ) and non-irradiated (AgNPs) silver particles synthesized by the alcoholic extract of bee propolis at different concentrations (10, 50, 100, 200 μg/l) on Chlorella vulgaris microalgae. The results demonstrated that exposure to AgNPs-γ and AgNPs and silver nitrate salts increased growth inhibition (%) in a dose-dependent manner (P<0.05). Moreover, cell viability (VC %), microalgal cell density/biomass (μ/L) and chlorophyll-a concentration decreased. In contrast, electrolyte conductivity/living cells (electrolyte leakage (EL %) increased in the exposed groups, compared with the control group (P<0.05). These results showed the following trend in inducing toxicity, inhibiting growth and photosynthesis in Chlorella vulgaris: AgNO3 < AgNPs < AgNPs-γ. | ||
| کلیدواژه‌ها [English] | ||
| Propolis, Silver Nanoparticles, Irradiation, Chlorella vulgaris | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 92 تعداد دریافت فایل اصل مقاله: 59 |
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