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Effective Dose Regimen of STZ for STZ-induced Diabetes in a Rat Model | ||
| Iranian Journal of Veterinary Medicine | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 06 تیر 1402 اصل مقاله (1016.07 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2023.358146.1005389 | ||
| نویسندگان | ||
| Olushola Adeleye* 1؛ Temitope Ajala1؛ Oluwatodimu Adewole Adekoya2؛ Adenike Iyabo Adeleye3 | ||
| 1Department of Veterinary Physiology and Biochemistry, Federal University of Agriculture, Abeokuta, Nigeria | ||
| 2Department of Veterinary Pharmacology and Toxicology, Federal University of Agriculture, Abeokuta, Nigeria | ||
| 3Veterinary Teaching Hospital, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. | ||
| چکیده | ||
| Diabetes mellitus (DM) is a metabolic illness, defined by elevated level of blood sugar because of a problem with insulin synthesis, action, or both. Various clinical signs follow DM, majorly hyperglycaemia, polydipsia, polyuria, and polyphagia. Worldwide prevalence is high and predicted to rise to 592 million by 2035. Animal models are used in the study of diabetes due to ethical issues. Streptozotocin (STZ) model is frequently used but has poor dependability due to unexplained acute toxicity and effective dose variability. This research was conducted to determine the effective dose regimen of STZ for inducing diabetes in Wister rats. 28 male Wistar rats (160 - 190 g) were randomly divided into 4 groups (n=7) and monitored for 21 days after diabetes induction with STZ: Control (CTR), diabetics: DIA1 (60 mg/kg STZ), DIA2 (60 mg/kg STZ twice at 0 and 24 hours), and DIA3 (60 mg/kg STZ thrice at 0, 24 and 48 hours). Plasma glucose was determined with a glucometer. Body weights, feed intake, and faecal output were weighed with a digital balance, while water intake and urine output were measured with a measuring cylinder. Analyses of data obtained were performed using a One-way ANOVA and Tukey’s test at p<0.05 for significance. There was significant (p<0.05) decrease in body weight of the diabetics (-15.53±1.2, -26.8±1.2, -28.5±1.9%) compared to the CTR (10.5±2.5%). There was significant (p<0.05) increase in fasting blood glucose concentrations (135.2±9.0, 273.2±6.5, 257.0±5.3 mg/dL) in the diabetics compared to the CTR (79.3±1.1 mg/dL). Water intake (56.9±0.9, 72.1±1.7, 77.8±5.5 mL), feed intake (19.4±0.6, 23.3±1.9, 42.1±2.1 g), voided urine (6.34±0.1, 8.39±0.88, 9.58±0.50 mL) and voided faeces (10.4±0.26, 11.7±0.43, 8.5±0.17 g) in the diabetics increased significantly (p<0.05) compared to the CTR (26.5 ± 0.8 mL, 13.4±0.3 g, 1.84±0.08 mL, and 6.5±0.33 g respectively). The dose regimen of 60 mg/kg STZ administered intraperitoneally twice (24 hours apart) sustained diabetes for 21 days. We recommend that this dose regimen be adopted in STZ-induced diabetic studies in male Wistar rats. | ||
| کلیدواژه‌ها | ||
| dose regimen؛ diabetes؛ streptozotocin؛ Wistar rats | ||
| عنوان مقاله [English] | ||
| رژیم دوز موثر STZ برای دیابت ناشی از STZ در مدل موش صحرایی | ||
| نویسندگان [English] | ||
| اولوشولا آدلیه1؛ تمی توپه آجالا1؛ اولوواتودیمو ادووله آدکویا2؛ آدنیک لیابو آدلیه3 | ||
| 1گروه فیزیولوژی دامپزشکی و بیوشیمی، دانشگاه فدرال کشاورزی، آبئوکوتا، نیجریه | ||
| 2گروه فارماکولوژی دامپزشکی و سم شناسی، دانشگاه فدرال کشاورزی، آبئوکوتا، نیجریه | ||
| 3بیمارستان آموزشی دامپزشکی، دانشگاه فدرال کشاورزی، آبئوکوتا، ایالت اوگون، نیجریه. | ||
| کلیدواژه‌ها [English] | ||
| رژیم دوز, دیابت, استرپتوزوتوسین, موش صحرایی ویستار | ||
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