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اثر دستکاری نسبت کربن به نیتروژن (C/N) بر عملکرد رشد، پاسخ ایمنی و شاخص استرس ماهی کپور معمولی (Cyprinus carpio) در مواجه حاد با استرس آمونیاک در سیستم بیوفلاک | ||
| شیلات | ||
| دوره 76، شماره 2، خرداد 1402، صفحه 265-278 اصل مقاله (1.11 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jfisheries.2023.353714.1365 | ||
| نویسندگان | ||
| بهزاد قولجایی1؛ حسین آدینه* 2؛ محمد هرسیج3؛ سیده آیناز شیرنگی4 | ||
| 1دانشجوی کارشناسی ارشد شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گلستان، ایران | ||
| 2استادیار گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گلستان، ایران | ||
| 3دانشیار گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گلستان، ایران | ||
| 4استادیار گروه زیست‌شناسی، دانشکده علوم پایه و فنی و مهندسی، دانشگاه گنبد کاووس، گلستان، ایران | ||
| چکیده | ||
| فناوری بیوفلاک یک فناوری جدید است که می‌تواند تولید آبزی‌پروری را بهبود بخشد و اهداف آبزی‌پروری پایدار را دنبال کند. در این آزمایش چهار سطح نسبت C/N بر کیفیت آب، عملکرد رشد، پاسخ ایمنی ماهی کپور معمولی (وزن اولیه 0/53±11/75 گرم) پرورش یافته در شرایط بیوفلاک بررسی شد. چهار سطح از نسبت های C/N، با استفاده از افزودن منبع کربن (شکر) ۱۰، ۱۵ و ۲۰ و شاهد بدون افزودن کربن (گروه شاهد با 50-40 درصد تعویض آب روزانه) از تیمارهای آزمایشی تشکیل شد. پس از 8 هفته، ماهیان به مدت 6 ساعت تحت استرس آمونیاک حاد (0/5 میلی گرم در لیتر) قرار گرفتند. قبل و بعد از مواجه با آمونیاک شاخص‌های استرس مانند گلوکز و کورتیزول سنجش و مورد مقایسة آماری قرار گرفتند. غلظت نیتروژن آمونیاکی کل (TAN) در C10 به‌طور معنی داری بالاتر بود. رشد ماهی در C15 به طور معنی داری بیشتر بود در حالی‌که ضریب تبدیل غذایی از نظر آماری تفاوت معنی داری نداشت. فعالیت های ایمونوگلوبولین، لیزوزیم و ACH50 سرم در گروه‌های C/N به طور معنی داری بالاتر از شاهد بود. شاخص های استرس (گلوکز و کورتیزول) قبل از قرار گرفتن در معرض تغییر معنی داری نشان ندادند، در حالی‌که پس از استرس آمونیاکی، سطح آن‌ها در همة گروه‌ها افزایش یافت و بالاترین سطح در گروه شاهد مشاهده شد. به طور کلی، محیط بیوفلاک (دستکاری C/N) اثر ضد استرس داشته و پرورش ماهی کپور معمولی در سیستم بیوفلاک با کمترین مقدار تعویض آب توصیه می‌شود. | ||
| کلیدواژه‌ها | ||
| ماهی کپور؛ بیوفلاک؛ آمونیاک؛ نشانگرهای استرس | ||
| عنوان مقاله [English] | ||
| Effect of carbon and nitrogen ratio (C:N) manipulation on growth performance, immune response and stress index of common carp (Cyprinus carpio) acute exposure to ammonia stress in biofloc system | ||
| نویسندگان [English] | ||
| Behzad Qoljaei1؛ Hossein Adineh2؛ Mohammad Harsij3؛ Seyedah Ainaz Shirangi4 | ||
| 1M.Sc. Student of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavos University, Golestan, Iran | ||
| 2Assistant Professor of Fisheries Department, Faculty of Agriculture and Natural Resources, Gonbad Kavos University, Golestan, Iran | ||
| 3Associate Professor of Fisheries Department, Faculty of Agriculture and Natural Resources, Gonbad Kavos University, Golestan, Iran | ||
| 4Assistant Professor of Biology Department, Faculty of Basic and Technical and Engineering Sciences, Gonbad Kavos University, Golestan, Iran | ||
| چکیده [English] | ||
| Biofloc is a novel technology that can improve aquaculture production and also pursue sustainable aquaculture goals. In this experiment, the impact of the different C/N ratios on water quality, growth performance and immune response of Cyprinus carpio (initial weight; 11.75±0.53g) reared under biofloc conditions were investigated. Four levels of C/N ratios, using the addition of a carbon source (sugar), 10, 15 and 20 and the control without adding carbon (Control group with 40-50% daily water exchange) were consisted of the experimental treatments. After 8 weeks, fish were subjected to acute ammonia stress (0.5mg/L) for 6 hrs. Stress indicators such as glucose and cortisol were measured and statistically compared before and after exposure to ammonia. Total ammonia nitrogen (TAN) concentration was significantly higher in C10. The growth of fish was significantly higher in C15 while the feed conversion ratio had no statistically significant difference. Serum immunoglobulin, lysozyme, ACH50 activities were significantly higher in the C/N groups than in the control. Stress indicators (glucose and cortisol) showed no significant change before exposure, while after ammonia stress, their levels increased in all groups and the highest level was observed in the control group. Overall, biofloc (C/N manipulation) had an anti-stress effect, and the culture of common carp in the biofloc system with the lowest daily water exchange could be recommended. | ||
| کلیدواژه‌ها [English] | ||
| Common carp, Biofloc, Ammonia, Stress biomarkers | ||
| مراجع | ||
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