Thermoacidophilic bacteria isolated from Sarcheshmeh low-grade copper ore in chalcopyrite bioleaching from mineral tailing | ||
| International Journal of Mining and Geo-Engineering | ||
| مقاله 4، دوره 56، شماره 4، زمستان 2022، صفحه 323-330 اصل مقاله (587.95 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22059/ijmge.2022.329157.594925 | ||
| نویسندگان | ||
| Fariba Heydarzadeh Sohi1؛ Abbas Akhavan Sepahi1؛ Fereshteh Rashchi* 2؛ Mohammad Kargar3؛ Seyed Abdolhamid Angaji4 | ||
| 1Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran. | ||
| 2School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, Tehran, Iran. | ||
| 3Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran. | ||
| 4Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. | ||
| چکیده | ||
| This research has focused on isolating and identifying different thermoacidophilic bacteria from a Sarcheshmeh low-grade copper ore and evaluating their ability of copper bioleaching from the mineral tailing. After the isolation of the bacteria, molecular identification was carried out based on the 16S rRNA gene sequences and drawing the phylogenetic tree. Then, the effect of the pH, pulp density, and composition of the media on the copper bioleaching was determined using the identified bacteria. The isolated strain (Strain SCM1) belonged to Delftia acidovorans with a 95.73% of identity. The optimal condition for the copper bioleaching was reported in a medium consisting of sulfur (10 g/L), glucose (10 g/L), yeast extract (2 g/L), and mineral tailing (5% wt/vol) at the pH of 2.00 at 50°C. Under this condition, the highest amount of copper (83%) was bioleached. It proves that the lately isolated strain can be effectively employed in the copper bioleaching process. | ||
| کلیدواژهها | ||
| Bioleaching؛ Copper؛ Mineral tailings؛ Thermoacidophilic bacteria؛ 16S rRNA | ||
| مراجع | ||
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