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تأثیر هیومیک‌اسید و تنش شوری بر برخی شاخص های جوانه زنی و مرفوفیزیولوژیکی گل‌راعی ایرانی در شرایط کشت بافت | ||
| به زراعی کشاورزی | ||
| مقاله 18، دوره 24، شماره 4، دی 1401، صفحه 1293-1310 اصل مقاله (592.3 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jci.2022.328340.2593 | ||
| نویسندگان | ||
| مهرداد رسولی* 1؛ علیرضا نوروزی شرف2 | ||
| 1نویسنده مسئول، گروه علوم و مهندسی باغبانی و فضای سبز، دانشگاه سیدجمال الدین اسدآبادی، اسدآباد، ایران. رایانامه: rasouli@sjau.ac.ir | ||
| 2گروه علوم و مهندسی باغبانی و فضای سبز، دانشگاه سیدجمال الدین اسدآبادی، اسدآباد، ایران. رایانامه: noroozi2ar@yahoo.com | ||
| چکیده | ||
| کاربرد ترکیبهایی که بتواند تحمل گیاهان را به تنشهای محیطی از جمله شوری افزایش دهد دارای اهمیت است. برای ارزیابی تعدیل تنش شوری با استفاده از هیومیک‌اسید آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار روی گیاه دارویی گل‌راعی بومی ایران توده همدان (Hypericum perforatum L.) در آزمایشگاه کشت بافت دانشکده کشاورزی دانشگاه سیدجمال الدین اسدآبادی در سال 1398 اجرا شد. تیمارهای آزمایشی شامل سه سطح شوری (صفر، 50 و 100 میلیمول بر لیتر) نمک کلریدسدیمکلریدسدیم و چهار سطح هیومیک‌اسید (صفر، 25، 50، 100 میلیگرم بر لیتر) بود. بیش‌ترین درصد (65/98 درصد) و سرعت جوانهزنی (94/38) و طول ریشه (21/34 میلی‌متر) در تیمار 50 میلیمول بر لیتر هیومیک‌اسید و بدون شوری بهدست آمد. بیش‌ترین ارتفاع گیاه، وزن تر و خشک اندام هوایی، وزن تر و خشک ریشه و کلروفیل کل مربوط به شرایط بدون تنش و هیومیک‌اسید با غلظت 100 میلیمول بر لیتر بود. بیش‌ترین مقدار فنل در تیمار شوری 50 میلیمولار و هیومیک‌اسید با غلظت 50 میلیگرم بر لیتر بود. بیش‌ترین میزان آنتی‌اکسیدان (77/99 میلیگرم بر گرم) و فلاونوئید کل (39/2 میلیگرم بر گرم) و کم‌ترین میزان پراکسیدهیدروژن (12/9 میکروگرم بر گرم) در تیمار شوری 100 میلیمولار و هیومیک‌اسید با غلظت 50 میلیگرم بر لیتر بهدست آمد. نتایج پژوهش نشان داد اثر تعدیل‌کننده بهویژه کاربرد سطوح 50 میلیگرم بر لیتر هیومیک‌اسید میتواند بر فرایندهای فیزیولوژیک و صفات رویشی گیاه گل‌راعی تحت تنش شوری اثرگذار باشد. | ||
| کلیدواژه‌ها | ||
| پراکسیداز؛ درصد جوانهزنی؛ فلاونوئید؛ کشت بافت؛ مواد آلی | ||
| عنوان مقاله [English] | ||
| Effects of Humic Acid and Salinity Stress on Some Germination and Morpho-physiological Indices of Iranian St Johns Wort in in Vitro Conditions | ||
| نویسندگان [English] | ||
| Mehrdad Rasouli1؛ Alireza Noroozisharaf2 | ||
| 1Corresponding Author, Department of Horticultural Science, Sayyed Jamaleddin Asadabadi University, Asadabad, Iran. E-mail: rasouli@sjau.ac.ir | ||
| 2Department of Horticultural Science, Sayyed Jamaleddin Asadabadi University, Asadabad, Iran. E-mail: noroozi2ar@yahoo.com | ||
| چکیده [English] | ||
| The use of compounds that can improve plant tolerance to abiotic environmental stress, including salinity, is important. To evaluate the adjustment of salinity stress using humic acid (HA), a factorial layout is conducted based on a complete randomized design with three replications on the Hypericum perforatum L. as an Iranian medicinal plant are collected from Hamadan province in the tissue culture laboratory of the Faculty of Agriculture, Sayyed Jamaleddin Asadabadi University during 2019. Experimental treatments include NaCl as salinity at three levels (such as 0, 50, and 100 mmol.L-1) and HA at four levels (control, 25, 50, and 100 mg.L-1). Results indicate that the plants treated with 50 mg.L-1 HA without salinity have had the highest germination percentage (98.65), germination rate (38.94) and root length (34.21 mm). The highest plant height, fresh and dry weight of both shoots and roots and total chlorophyll are obtained under control conditions and HA with a concentration of 100 mg.L-1. On the contrary, the highest amount of phenol in the treatment is 50 mmol.L-1 salinity and HA with a concentration of 50 mg.L-1. In the treatment of 100 mmol.L-1 salinity and HA with a concentration of 50 mg.L-1, the highest amount of total antioxidants (99.77 mg/g FW) and total flavonoids (2.39 mg/Qe g FW) and the lowest amount of hydrogen peroxide (9.12 µg/g FW) are obtained. Results show that the mitigating effect, especially the application of HA of 50 mg.L-1 levels, can affect the physiological processes and morphological traits of the Hypericum perforatum L. under salinity stress. | ||
| کلیدواژه‌ها [English] | ||
| Flavonoids, Germination percentage, Organic matter, Peroxidase, Tissue culture | ||
| مراجع | ||
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