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بررسی فعالیت آنزیمهای آنتیاکسیدان و پاسخهای بیوشیمیایی گیاه گلرنگ تحت تأثیر محلولپاشی ملاتونین در شرایط تنش خشکی | ||
به زراعی کشاورزی | ||
مقاله 14، دوره 23، شماره 4، دی 1400، صفحه 906-883 اصل مقاله (740.3 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jci.2021.315815.2490 | ||
نویسندگان | ||
سیاوش حشمتی* 1؛ غلام عباس اکبری2؛ الیاس سلطانی3؛ مجید امینی دهقی4؛ کیوان فتحی امیرخیز5؛ کیوان ملکی6 | ||
1دانشآموخته دکتری، گروه علوم زراعی و اصلاح نباتات، دانشکدگان ابوریحان، دانشگاه تهران، پاکدشت، ایران. | ||
2دانشیار، گروه علوم زراعی و اصلاح نباتات، دانشکدگان ابوریحان، دانشگاه تهران، پاکدشت، تهران، ایران. | ||
3دانشیار، گروه علوم زراعی و اصلاح نباتات، دانشکدگان ابوریحان، دانشگاه تهران، پاکدشت، ایران. | ||
4دانشیار، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی دانشگاه شاهد، تهران، ایران. | ||
5دانشجوی دکتری زراعت، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران | ||
6دانش آموخته کارشناسی ارشد زراعت، گروه علوم زراعی و اصلاح نباتات، دانشکدگان ابوریحان، دانشگاه تهران، پاکدشت، تهران | ||
چکیده | ||
به منظور بررسی تأثیر محلولپاشی ملاتونین بر گیاهان گلرنگ رشد یافته از بذور با کیفیت مختلف گلرنگ در شرایط تنش خشکی، دو آزمایش مزرعهای طی سالهای زراعی 96-1395 و 97-1396 در مزرعه پژوهشی پردیس ابوریحان دانشگاه تهران انجام گرفت. طرح آزمایشی بهصورت اسپلیت-فاکتوریل در قالب طرح بلوکهای کامل تصادفی با چهار تکرار انجام گرفت. عامل اصلی شامل دو سطح تنش خشکی 1- بدون تنش و 2- تنش (بهترتیب آبیاری پس از 50 و 85 درصد تخلیه رطوبت از ظرفیت زراعی خاک در مرحله گلدهی) بود که در کرتهای اصلی قرار گرفتند. کرتهای فرعی، شامل چهار تیمار بودند که عبارت بودند از از کیفیت بذر (بذور انبارشده و بذور تازه برداشتشده) و محلولپاشی (1- شاهد و 2- محلولپاشی ملاتونین). نتایج این آزمایش نشان داد محلولپاشی ملاتونین عملکر دانه در بذور انبارشده را به مقدار 589 کیلوگرم در هکتار نسبت به تیمار محلولپاشی با آب (شاهد) افزایش داد. نتایج نشان داد که فعالیت آنزیمهای سوپراکسیددیسموتاز و کاتالاز در گیاهان حاصل از بذور تازه برداشتشده در پاسخ به محلولپاشی ملاتونین در شرایط تنش خشکی افزایش یافت. همچنین مشخص شد که در گیاهان حاصل از بذور تازه برداشتشده، محلولپاشی با ملاتونین، میزان پروتئینهای محلول برگ را تا 28 درصد کاهش داد. نتایج مشخص کرد که در گیاهان حاصل از بذور انبارشده، محلولپاشی ملاتونین، میزان مالوندیآلدهید را در شرایط تنش خشکی، به میزان 37 درصد کاهش داد. بهنظر میرسد کیفیت بذر مورداستفاده در ویژگیهای گیاهان حاصل از آن بذرها تأثیر داشته باشد و همچنین استفاده از ملاتونین در شرایط تنش خشکی، ممکن است نقش قابلتوجهی در کاهش شدت آسیب حاصل از تنش روی گلرنگ داشته باشد. | ||
کلیدواژهها | ||
پرولین؛ دانه روغنی؛ عملکرد دانه؛ کاتالاز؛ مالون دآلدهید | ||
مراجع | ||
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