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تأثیر حاد تمرین تناوبی با شدت بالا (HIIT) بر بیان ژن‌های FNDC5 و PGC-1α در رت‌های نر دیابتی | ||
| نشریه علوم زیستی ورزشی | ||
| مقاله 1، دوره 12، شماره 3، آذر 1399، صفحه 249-261 اصل مقاله (348.07 K) | ||
| نوع مقاله: مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I | ||
| شناسه دیجیتال (DOI): 10.22059/jsb.2020.119643.902 | ||
| نویسندگان | ||
| موسی خلفی* 1؛ علی اصغر رواسی2؛ رحمان سوری3؛ محمد مرادی4؛ مسعود سلیمانی5 | ||
| 1دکتری فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران | ||
| 2. استاد گروه فیزیولوژی ورزشی، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران | ||
| 3استاد گروه فیزیولوژی ورزشی، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران | ||
| 4. دانشجوی دکتری فیزیولوژی ورزشی، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران | ||
| 5دانشیار دانشگاه تربیت مدرس، تهران، ایران | ||
| چکیده | ||
| بیان PGC-1α ناشی از فعالیت ورزشی به افزایش بیان FNDC5 در عضله منجر می‌شود، این پروتئین غشایی هورمون تازه شناسایی‌شدۀ ایرزین را ترشح می‌کند که سبب افزایش انرژی مصرفی، بهبود حساسیت انسولینی و تحمل گلوکز می‌شود. هدف از پژوهش حاضر بررسی تأثیر حاد تمرین تناوبی با شدت بالا (HIIT) بر بیان ژن‌های FNDC5 و PGC-1α رت‌های نر دیابتی است. در این مطالعه 18 سر رت نر دیابتی (12 هفته سن و با وزن 240-220 گرم) به سه گروه تقسیم شدند: تمرین تناوبی با شدت بالا بلافاصله (HIIT0)(6سر)، تمرین تناوبی با شدت بالا 2 ساعت بعد (HIIT2) (6سر) و کنترل (C) (5سر). هر دو گروه HIIT با سرعت vo2max90-95% در 12 تناوب 1 دقیقه‌ای با فواصل استراحتی 1 دقیقه‌ای به فعالیت روی نوار گردان پرداختند. برای بررسی بیان نسبی mRNA ژن‌های FNDC5 و PGC-1α بافت عضلانی از روش Real time PCR استفاده شد. از آزمون ANOVA و تست تعقیبی توکی برای تحلیل داده‌ها استفاده و سطح معنا‌داری 05/0 درنظر گرفته شد. تحلیل داده‌ها نشان داد که بین گروه‌های تحقیق در بیان ژن‌های FNDC5 و PGC-1α تفاوت معنا‌داری وجود دارد (01/0P≤). نتایج آزمون توکی نشان داد، بیان ژن‌های FNDC5 و PGC-1α در هر دو گروه تمرین تناوبی با شدت بالا (HIIT0-HIIT2) نسبت به گروه کنترل افزایش معنا‌داری داشتند (01/0P≤). بنابراین، نتایج این تحقیق نشان داد که یک جلسه تمرین تناوبی با شدت بالا (HIIIT) با القای PGC-1α به تحریک بیان ژن FNDC5 در رت‌های دیابتی منجر می‌شود. | ||
| کلیدواژه‌ها | ||
| بیان ژن FNDC5؛ بیان ژن PGC-1α؛ تمرین تناوبی با شدت بالا (HIIT)؛ رت‌های دیابتی | ||
| عنوان مقاله [English] | ||
| The Acute Effects of High Intensity Interval Training (HIIIT) on PGC-1α and FNDC5 Genes Expression in Diabetic Rats | ||
| نویسندگان [English] | ||
| Mousa Khalafi1؛ Ali Asghar Ravasi2؛ Rahman Soori3؛ Mohammad Moradi4؛ Massoud Soleimani5 | ||
| 1PhD of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran | ||
| 2Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran, | ||
| 3Professor, Department of Exercise Physiology, Faculty of Physical Education, University of Tehran, Tehran, Iran | ||
| 4PhD Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran | ||
| 5Associate Professor, Tarbiat Modares University, Tehran, Iran | ||
| چکیده [English] | ||
| Change in adipose tissue phenotype caused by exercise training is new theory that as result of muscle FNDC5 gene expression. The aim of the present study is to investigate the acute effects of high intensity interval training (HIIT( on PGC-1α and FNDC5 genes expression of diabetic rats. In this study, 18 diabetic wistar rats(12 week- age, 220-240 gr- weight) were assigned to three groups: high intensity interval training Immediately (HIIE0)(n=6), high intensity interval training 2hours later (HIIE2)(n=6) and control(C)(n=5). Both HIIT groups activated on the treadmill with 90-95% vo2max in the 12 interval-one minute period and 1 minute rest intervals. Real time PCR method was used for the relative expression of mRNA FNDC5 and PGC-1α. One-way ANOVA and Tukey Post hoc test has used to data analysis, the level of significance has been consider at 0/05. Data Analysis showed significant differences Research groups in expression of mRNA FNDC5 and PGC-1α (p≤0/01.) Tukey test showed, FNDC5 and PGC-1α gene expression In the 2 groups high intensity interval training(HIIT0-HIIT2) compared to the control group significantly increased (p≤0/01). | ||
| کلیدواژه‌ها [English] | ||
| high intensity interval training (HIIIT), FNDC5 gene expression, PGC-1α gene expression, diabetic rats | ||
| مراجع | ||
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