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بررسی اثر برخی عوامل زنده و غیر زنده القا کننده مقاومت بر فعالیت آنزیمی و فاکتورهای رشدی لوبیا در حضور ویروس موزاییک زرد لوبیا | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| دوره 9، شماره 1، شهریور 1399، صفحه 46-60 اصل مقاله (863.3 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2020.298097.288 | ||
| نویسندگان | ||
| محمد زارعی1؛ احمد حسینی* 2؛ روح اله صابری3 | ||
| 1دانشجوی سابق کارشناسی ارشد گروه گیاه پزشکی- دانشکده کشاورزی- دانشگاه ولی عصر (عج) رفسنجان | ||
| 2گروه گیاهپزشکی، دانشگاه ولی عصر (عج) رفسنجان | ||
| 3دانشیار گروه گیاهپزشکی دانشکده کشاورزی دانشگاه ولی عصر (عج) رفسنجان | ||
| چکیده | ||
| ویروس موزاییک زرد لوبیا (BYMV) متعلق به جنس پوتی‌ویروس(Potyvirus) ، قادر به آلوده کردن طیف وسیعی از گیاهان میباشد. این ویروس از طریق مکانیکی و شته به‌صورت ناپایا انتقال می‌یابد. در این پژوهش تاثیر شش تیمار شامل نانو ذرات نقره سنتز شده توسط سویه‌ی CHA0 باکتریP. fluorescens ، سویه 62 قارچ T. harizianum، باکتری P. fluorescens CHA0، عصاره الکلی گیاهان زیتون (Olea europaea) و بومادران (Achillea millefolium) و فسفیت پتاسیم در کاهش خسارت یک سویه‌ِی BYMV در سه ژنوتیپ لوبیا (KS21189، اختر و پاک) مورد بررسی قرار گرفت. این آزمایش به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی انجام شد. برای انجام این پژوهش ابتدا گیاهان به‌صورت مکانیکی با یک سویه‌ی BYMV مایه‌زنی شدند. همزمان با ظهور علائم ویروسی، تیمارهای مختلف روی گیاهان اعمال شد. غلظت ویروس در گیاهان تیمار شده با آزمون DAS-ELISA و بهرهگیری از آنتیسرم چندهمسانه ای BYMV مورد ارزیابی قرار گرفت. ارزیابی پارامترهای رویشی و زایشی، میزان فعالیت آنزیم پراکسیداز و غلظت ویروس در سه ژنوتیپ لوبیا نسبت به BYMV نشان داد که بیشترین تاثیر بر کاهش غلظت ویروس موزاییک زرد لوبیا، مربوط به تیمارهای فسفیت پتاسیم ، عصاره الکلی بومادران و باکتری Pseudomonas fluorescens CHA0 بوده است. | ||
| کلیدواژه‌ها | ||
| ویروس موزاییک زرد لوبیا؛ مقاومت القایی؛ باکتریP. fluorescens؛ تریکودرما و فسفیت پتاسیم | ||
| عنوان مقاله [English] | ||
| Study of Effect of some biotic and abiotic treatments inducing resistance on enzymatic activity and growth factors in common bean infected with Bean yellow mosaic virus | ||
| نویسندگان [English] | ||
| Mohammad Zarei1؛ Ahmad Hosseini2؛ Roohallah Saberi riseh3 | ||
| 1Department of Plant Protection Vail-e-Asr University of Rafsanjan Iran | ||
| 2Department of Plant Protection, Vali-e-Asr University of Rafsanjan, iran | ||
| 3Department of Plant Protection Vail-e-Asr University of Rafsanjan, Iran | ||
| چکیده [English] | ||
| Bean yellow mosaic virus (BYMV) which belongs to Potyvirus genus, is able to infect wide range of plants because of its wide host range. This virus is transmitted by mechanical inoculation and aphid vectors in non-persistent manner. In this research the effects of six treatments including: synthesized silver nano particles of P. fluorescens CHA0, preparations of T. harizianum and P. fluorescens CHA0, olive (Olea europaea) and yarrow (Achillea millefolium) extracts and potassium phosphite on damage reduction in three common bean genotypes against a BYMV isolate was investigated. This experiment was carried out in a completely randomized factorial design. For this purpose, treatments were applied on plants immediately afterward first symptoms in mechanically inoculated plants were appeared. Virus concentration in treated plants was evaluated by DAS-ELISA test using BYMV polycolonal antibody. Evaluation of vegetative and reproductive parameters, measurement of peroxidase and polyphenol oxidase enzymes activity and virus accumulation indicated that potassium phosphite, silver nano particles of P. fluorescens CHA0 and yarrow extracts had highest influence on virus accumulation | ||
| کلیدواژه‌ها [English] | ||
| Bean yellow mosaic virus, Trichoderma, Potassium phosphite and Induced resistance | ||
| مراجع | ||
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