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بررسی تغییرات محتوای پروتئینCas3، فیبرونکتین و Col4a1 در پی دو شیوۀ تمرین هوازی و اینتروال همراه با مصرف مکمل آستاگزانتین در بافت قلب رتهای دیابتی نوع2 | ||
نشریه علوم زیستی ورزشی | ||
مقاله 6، دوره 12، شماره 4، بهمن 1399، صفحه 453-471 اصل مقاله (456.97 K) | ||
نوع مقاله: مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I | ||
شناسه دیجیتال (DOI): 10.22059/jsb.2020.306858.1414 | ||
نویسندگان | ||
نسرین عبادی1؛ محمدرضا ذوالفقاری* 2؛ فیروز قادری پاکدل3 | ||
1دانشآموختۀ دکتری فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی، دانشگاه ارومیه، ارومیه، ایران | ||
2استادیار فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی، دانشگاه ارومیه، ارومیه، ایران | ||
3دانشیار فیزیولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران | ||
چکیده | ||
دیابت بهعنوان نوعی اختلال متابولیک مزمن با شیوع گسترده، اهمیت تحقیقات مداوم و نیاز به روشهای نوین را برای پیشگیری و درمان این بیماری همهگیر برجسته میکند. هدف از تحقیق حاضر بررسی تغییرات فیبروزی و آپوپتوزی بافت قلبی در رتهای دیابتی در پی اجرای دو شیوۀ تمرینی هوازی و اینتروال با مصرف مکمل آستاگزانتین بود. 35 سر موش صحرایی نر پس از القای دیابت بهطور تصادفی در 7گروه شامل دیابتی کنترل، دیابتی شم، دیابت+ هوازی+ مکمل، دیابت + اینترول+ مکمل، دیابت+اینتروال، دیابت+ هوازی، دیابت+ مکمل قرار گرفتند.تمرینات اینتروال به مدت 8 هفته با 5 جلسه در هفته با شدت 80 درصد Vo2max و تمرینات هوازی با شدت 65 تا 75 درصد Vo2max روی نوار گردان اجرا شد. محتوای پروتئین Cas3، COL4a1 و فیبرونکتین با تکنیک وسترن بلات اندازهگیری شد. محتوای پروتئینCOL4a1 در هر دو گروه تمرینی و ترکیبی و گروه مکمل کاهش معناداری نسبت به گروه کنترل نشان داد. محتوای پروتئین Cas3 و فیبرونکتین گروههای ترکیبی و هوازی نیز نسبت به گروه کنترل کاهش معناداری داشت، اما در مقایسۀ محتوای این پروتئینها بین گروههای مکمل و اینتروال نسبت به گروه کنترل اختلاف معناداری وجود نداشت. بهنظر میرسد تأثیرات همافزای تمرینات ورزشی با شدتهای مختلف همراه با مکملسازی آستاگزانتین از روند صعودی آپوپتوز و فیبروز در بافت قلبی رتهای دیابتی و عوارض متعاقب آن همچون کاردیومیوپاتی دیابتی پیشگیری میکند. | ||
کلیدواژهها | ||
آستاگزانتین؛ تمرین؛ دیابت؛ رت؛ کاردیومیوپاتی دیابتی | ||
مراجع | ||
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