تعداد نشریات | 161 |
تعداد شمارهها | 6,532 |
تعداد مقالات | 70,501 |
تعداد مشاهده مقاله | 124,098,990 |
تعداد دریافت فایل اصل مقاله | 97,206,555 |
Isolation of lipase producing bacteria from olive extract to improve lipase production using a submerge fermentation technique | ||
Journal of Food and Bioprocess Engineering | ||
مقاله 1، دوره 1، شماره 1، شهریور 2018، صفحه 1-6 اصل مقاله (381.58 K) | ||
نوع مقاله: Original research | ||
نویسندگان | ||
Samira Moradi؛ Seyed Hadi Razavi* ؛ Seyed Mohammad Mousavi | ||
Bioprocess Engineering Laboratory (BPEL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran | ||
چکیده | ||
The tremendous mounting interest in lipase production is pertinent to the biotechnological applications that these enzymes offer. Lipase, belonging to the hydrolyze enzymes, is involved in copious biological functions including: hydrolysis insoluble triacylglycerol to mono and diacylglycerols and glycerol. Submerge fermentation technique was applied for the efficient production of this. Briefly, olive oil emulsified by Arabic gum (3% olive oil with 8% Arabic gum) and coriander seed extract (7%) were used as supplementation. Extract was isolated from the olive used to isolation lipase producing bacteria. Among the isolates, mesophilic OE3 strain was selected as the best lipase producing bacterium with high hydrolytic activities (663U/ml). From Rhodamin B, we applied agar plate technique to screen lipase production bacteria based on e formation of the fluorescence halo around the colony under radiation of UV. The isolate was short rod- and coccoid-like. The isolate was Gram-positive, aerobic, non-motile, non-endospore-forming, non-acid fast, oxidase-negative, and catalase-positive. The selected isolate was positive for H2S and indole production and urea hydrolysis; however, they could not reduce nitrates to nitrogen. The final achievement was a lipase activity 2645 U/ml after 48 h incubation in 37ºC. | ||
کلیدواژهها | ||
Lipase enzyme؛ Olive extract؛ Submerge fermentation؛ Hydrolytic activities | ||
مراجع | ||
Abdel-Fattah, Y. R., Soliman, N. A., Yousef, S. M., & El-Helow, E. R. (2012). Application of experimental designs to optimize medium composition for production of thermostable lipase/esterase by Geobacillus thermodenitrificans AZ1. Journal of Genetic Engineering and Biotechnology, 10(2), 193-200.
Burkert, J. F. M., Maugeri, F., & Rodrigues, M. I. (2004). Optimization of extracellular lipase production by Geotrichum sp. using factorial design. Bioresource technology, 91(1), 77-84.
Carrière, F., Thirstrup, K., Hjorth, S., Ferrato, F., Nielsen, P. F., Withers-Martinez, C., ... & Verger, R. (1997). Pancreatic lipase structure− function relationships by domain exchange. Biochemistry, 36(1), 239-248.
Castro-Ochoa, L. D., Rodríguez-Gómez, C., Valerio-Alfaro, G., & Ros, R. O. (2005). Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme and Microbial Technology, 37(6), 648-654.
Chen, S. J., Cheng, C. Y., & Chen, T. L. (1998). Production of an alkaline lipase by Acinetobacter radioresistens. Journal of fermentation and bioengineering, 86(3), 308-312.
Colla, L. M., Rizzardi, J., Pinto, M. H., Reinehr, C. O., Bertolin, T. E., & Costa, J. A. V. (2010). Simultaneous production of lipases and biosurfactants by submerged and solid-state bioprocesses. Bioresource technology, 101(21), 8308-8314.
Dheeman, D. S., Henehan, G. T., & Frías, J. M. (2011). Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis. Bioresource technology, 102(3), 3373-3379.
Dominguez, A., Costas, M., Longo, M. A., & Sanromán, A. (2003). A novel application of solid state culture: production of lipases by Yarrowia lipolytica. Biotechnology Letters, 25(15), 1225-1229.
Fadı́loğlu, S., & Erkmen, O. (2002). Inactivation of lipase by carbon dioxide under atmospheric pressure. Journal of food engineering, 52(4), 331-335.
Gharibzahedi, S. M. T., Razavi, S. H., & Mousavi, S. M. (2013). Ultrasound-assisted formation of the canthaxanthin emulsions stabilized by arabic and xanthan gums. Carbohydrate polymers, 96(1), 21-30.
Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., & Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology (9th edn.), Baltimore, Philadelphia, Hong Kong.
Hou, C. T., & Johnston, T. M. (1992). Screening of lipase activities with cultures from the agricultural research service culture collection. Journal of the American Oil Chemists’ Society, 69(11), 1088-1097.
Houde, A., Kademi, A., & Leblanc, D. (2004). Lipases and their industrial applications. Applied biochemistry and biotechnology, 118(1-3), 155-170.
Jaeger, K. E., Dijkstra, B. W., & Reetz, M. T. (1999). Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases. Annual Reviews in Microbiology, 53(1), 315-351.
Joseph, B., Ramteke, P. W., Thomas, G., & Shrivastava, N. (2007). Cold-active microbial lipases: a versatile tool for industrial applications. Biotechnology and Molecular Biology Reviews, 2(2), 39-48.
Joseph, B., Shrivastava, N., & Ramteke, P. W. (2012). Extracellular cold-active lipase of Microbacterium luteolum isolated from Gangotri glacier, western Himalaya: Isolation, partial purification and characterization. Journal of Genetic Engineering and Biotechnology, 10(1), 137-144.
Kempka, A. P., Lipke, N. L., Pinheiro, T. D. L. F., Menoncin, S., Treichel, H., Freire, D. M., ... & de Oliveira, D. (2008). Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid-state fermentation. Bioprocess and Biosystems Engineering, 31(2), 119-125.
Liu, C. H., Chen, C. Y., Wang, Y. W., & Chang, J. S. (2011). Fermentation strategies for the production of lipase by an indigenous isolate Burkholderia sp. C20. Biochemical engineering journal, 58, 96-102.
Liu, C. H., Huang, C. C., Wang, Y. W., & Chang, J. S. (2012). Optimizing lipase production from isolated Burkholderia sp. Journal of the Taiwan Institute of Chemical Engineers, 43(4), 511-516.
Ruchi, G., Anshu, G., & Khare, S. K. (2008). Lipase from solvent tolerant Pseudomonas aeruginosa strain: Production optimization by response surface methodology and application. Bioresource technology, 99(11), 4796-4802.
Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Production, purification, characterization, and applications of lipases. Biotechnology advances, 19(8), 627-662.
Silva, W. O. B., Mitidieri, S., Schrank, A., & Vainstein, M. H. (2005). Production and extraction of an extracellular lipase from the entomopathogenic fungus Metarhizium anisopliae. Process Biochemistry, 40(1), 321-326.
Tayyab, M., N. Rashid and M. Akhtar (2011). Isolation and identification of lipase producing thermophilic Geobacillus sp SBS-4S: Cloning and characterization of the lipase. Journal of bioscience and bioengineering 111(3): 272-278.
Winkler, U. K., & Stuckmann, M. A. R. T. I. N. A. (1979). Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. Journal of bacteriology, 138(3), 663-670. | ||
آمار تعداد مشاهده مقاله: 325 تعداد دریافت فایل اصل مقاله: 361 |