Tarakemeh, Ameneh, Azizi, Majid, Rowshan, Vahid, Salehi, Hassan, Spina, Rosella, Dupire, François, Arouei, Hossein, Laurain-Mattar, Dominique. (1398). Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS. , 6(2), 151-157. doi: 10.22059/ijhst.2019.280853.295
Ameneh Tarakemeh; Majid Azizi; Vahid Rowshan; Hassan Salehi; Rosella Spina; François Dupire; Hossein Arouei; Dominique Laurain-Mattar. "Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS". , 6, 2, 1398, 151-157. doi: 10.22059/ijhst.2019.280853.295
Tarakemeh, Ameneh, Azizi, Majid, Rowshan, Vahid, Salehi, Hassan, Spina, Rosella, Dupire, François, Arouei, Hossein, Laurain-Mattar, Dominique. (1398). 'Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS', , 6(2), pp. 151-157. doi: 10.22059/ijhst.2019.280853.295
Tarakemeh, Ameneh, Azizi, Majid, Rowshan, Vahid, Salehi, Hassan, Spina, Rosella, Dupire, François, Arouei, Hossein, Laurain-Mattar, Dominique. Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS. , 1398; 6(2): 151-157. doi: 10.22059/ijhst.2019.280853.295

Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS

International Journal of Horticultural Science and Technology
مقاله 1، دوره 6، شماره 2، اسفند 2019، صفحه 151-157    اصل مقاله (696.39 K)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2019.280853.295
نویسندگان
Ameneh Tarakemeh1؛ Majid Azizi* 1؛ Vahid Rowshan2؛ Hassan Salehi3؛ Rosella Spina4؛ François Dupire5؛ Hossein Arouei1؛ Dominique Laurain-Mattar4
1Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad, Iran.
2Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.
3Department of Horticultural Science, Shiraz University, Shiraz, Iran.
4Université de Lorraine, CNRS, L2CM, F-54000 Nancy, France.
5Université de Lorraine, CNRS, L2CM, F-54000 Nancy, France. Université de Lorraine, Service Commun de Spectrométrie de Masse, F-54000 Nancy, France.
چکیده
Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a widespread species, native to the Mediterranean region with considerable distribution in Iran. In the present study, calli, roots and bulblets were regenerated from N. tazetta var. Meskin. In order to report alkaloid profile and quantify galanthamine and lycorine, methanolic extract of different in vitro tissues were subjected to alkaloid purification by SPE (solid phase extraction) method, followed by GC-MS analysis. In general, galanthamine and lycorine were detected in all in vitro tissues, while demethylmaritidine was only identified in bulblets. Bulblet was identified as the best source of main Amaryllidaceae alkaloids regarding the highest quantities of galanthamine and lycorine with 15 and 731 µg/g dry weight (DW) respectively.
کلیدواژه‌ها
Amaryllidaceae alkaloids؛ Galanthamine؛ Lycorine؛ Narcissus؛ Tissue culture
مراجع
آمار
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