تعداد نشریات | 161 |
تعداد شمارهها | 6,572 |
تعداد مقالات | 71,021 |
تعداد مشاهده مقاله | 125,497,702 |
تعداد دریافت فایل اصل مقاله | 98,759,208 |
Assessing Genetic Diversity of Shishi Date Palm Cultivars in Saudi Arabia and Qatar Using Microsatellite Markers | ||
International Journal of Horticultural Science and Technology | ||
مقاله 1، دوره 6، شماره 1، شهریور 2019، صفحه 1-9 اصل مقاله (668.46 K) | ||
نوع مقاله: Research paper | ||
شناسه دیجیتال (DOI): 10.22059/ijhst.2019.271662.267 | ||
نویسندگان | ||
Khaled Elmeer* 1؛ Imene Mattat2؛ Ameena Al-Malki2؛ Al-Ghaliya Al-Mamari3؛ Khaled BoJulaia4؛ Aladdin Hamwieh5؛ Michael Baum5 | ||
1Horticulture Department, Faculty of Agriculture, University of Tripoli, Libya | ||
2Genetic Engineering, Department of Agricultural Research, Ministry of Municipality and Environment, Doha Qatar | ||
3Biotechnology Research Section, Ministry of Agriculture and Fisheries Sultanate of Oman | ||
4Ministry of Agriculture, King Saudi Arabia | ||
5International Center for Agricultural Research in the Dry Areas, (ICARDA), P. O. Box: 2416, Cairo, Egypt | ||
چکیده | ||
In this study fourteen microsatellite primer pairs were used to study the genetic diversity of Shishi Date palm in Qatar. A total of 32 date palm (15 Shishi cultivar, 10 Khalas and seven male date palms) were collected from Qatar and 5 Shihi cultivars were collected from Saudi Arabia for comparison. The Shishi set collected from Qatar was selected from different regions to represent the genetic diversity of this cultivar. The results indicated 98 alleles produced from the 14 microsatellite markers, and the cluster analysis showed four major clusters corresponding o the geographical areas. Similarly, the structure analysis indicated four populations according to statistic K value. PCoA analysis showed three groups (A, B and C) separating Shishi (from Qatar) in group A, Khalas in group B and Shishi (from Saudi Arabia) in group C and no clear group separated the male genotypes. This indicates that the sexual propagation by seeds is the main source of variation in the date palm. This is the first study focusing on Shishi cultivar in Qatar and Saudi Arabia by using molecular markers. | ||
کلیدواژهها | ||
Date palm؛ Khalas؛ Microsatellite؛ Phoenix dactylifera؛ Shihi | ||
مراجع | ||
10. Hamza H, Abederrahim M.A.B, Elbekkay M, Ferchichi A. 2012. Comparison of the effectiveness of ISSR and SSR markers in determination of date palm (Phoenix dactylifera L.) agronomic traits. Australian Journal of Crop Science 7(6), 763. 11. Jarvis D, Padoch C, Cooper H. 2007. Biodiversity, Agriculture, and Ecosystem Services. Managing biodiversity in agricultural ecosystems. Columbia University Press. 12. Liu K, Muse S.V. 2005. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21, 2128-2129. 13. Peakall R, Smouse P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6, 288-295. 14. Popenoe P.B. 1973. The date palm. Field Research Projects, Coconut Grove, Miami. 15. Pritchard J.K, Stephens M, Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics 155(2), 945-959. 16. Rogers S.O, Bendich A.J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant molecular biology 5(2), 69-76. 17. Shah J. 2014. Date palm cultivation in India: an overview of activities. Emirates Journal of Food and Agriculture 26, 987-999. 18. Torres A.M, Tisserat B. 1980. Leaf isozymes as genetic markers in date palms. American Journal of Botany 67(2), 162-167. 19. Yeh F.C, Yang R.C, Boyle T.B.J, Ye ZH, Mao J.X. 1997. Popgene, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta. 20. Yusuf A, Culham A, Aljuhani W, Ataga C, Hamza A, Odewale J, Enaberue L. 2015. Genetic Diversity of Nigerian Date Palm (Phoenix dactylifera) Germplasm based on Microsatellite Markers. International Journal of Bio-Science and Bio-Technology 7, 121-132. 21. Zaid A, De Wet P.F. 2002. Botanical and Systematic Description of Date Palm. FAO Plant Production and Protection Paper. Date Palm Cultivation 156(1), 14-16. 22. Zehdi S, Sakka H, Rhouma A, Salem A.O.M, Marrakchi M, Trifi M. 2004. Analysis of Tunisian date palm germplasm using simple sequence repeat primers. African journal of biotechnology 3, 215-219. | ||
آمار تعداد مشاهده مقاله: 833 تعداد دریافت فایل اصل مقاله: 684 |