Quantitative Assessment of Diosgenin from Different Ecotypes of Iranian Fenugreek (Trigonella foenum-graecum L.) by High-performance Liquid Chromatography | ||
| International Journal of Horticultural Science and Technology | ||
| مقاله 10، دوره 5، شماره 1، شهریور 2018، صفحه 103-109 اصل مقاله (573.69 K) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.22059/ijhst.2018.254988.230 | ||
| نویسندگان | ||
| Sahar Aminkar1؛ Abdolali Shojaeiyan* 2؛ Sajad Rashidi Monfared3؛ Mahdi Ayyari2 | ||
| 1Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. | ||
| 3Department of Plant Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. | ||
| چکیده | ||
| In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (Trigonella foenum-graecum) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g-1dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g-1dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry. | ||
| کلیدواژهها | ||
| Dioscin؛ HPLC؛ Quantitative determination؛ Secondary metabolites؛ Steroidal saponin | ||
| مراجع | ||
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