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Molecular evaluation of M2 protein of Iranian avian influenza viruses of H9N2 subtype in order to find mutations of adamantane drug resistance | ||
| Iranian Journal of Veterinary Medicine | ||
| مقاله 2، دوره 10، شماره 4، اسفند 2016، صفحه 253-262 اصل مقاله (1.48 M) | ||
| نوع مقاله: Infectious agents- Diseases | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2016.59718 | ||
| نویسندگان | ||
| Mohammad Malekan1؛ Mehdi VasfiMarandi* 2؛ abas Barin3؛ Talat Mokhtari azad4؛ Mohammad Mehdi Ranjbar3؛ Mohsen Bashashati5 | ||
| 11Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 2Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 3Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 4Department of Virology, School of Public Health, Tehran University of Medical Science, Tehran, Iran | ||
| 5Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Background: The H9N2 subtype of influenza A viruses is considered to be widespread in poultry industry. Adamantane is a group of antiviral agents which is effective both in prevention and treatment of influenza A virus infections. These drugs inhibit M2 protein ion channel which has role on viral replication. OBJECTIVES: The main objective of this study is to evaluate M gene of avian influenza viruses (AIVs) of H9N2 subtype in order to find adamantane drug resistance mutations. METHODS: Over 100 suspected samples were collected from different geographical regions of Iran during 2012-2013. Samples were injected via allantoic sac of 9-11 day-old chicken embryos. A total of 11 out of 100 were AIV. The H9N2 subtype was confirmed by specific RT-PCR. The RT-PCR was conducted for full length M gene. PCR amplified products were purified and then conducted for commercial direct sequencing. Finally, sequences were checked for possible sites of adamantane resistance mutations. RESULTS: Overall, 8 out of 11 viruses harbored the adamantane resistance-associated mutations. Of which, four viruses were isolated in 2013 and four viruses in 2012. Two different resistance-associated mutations were observed during different years. CONCLUSIONS: The present study provided clear evidence concerning resistance AIVs of H9N2 subtype that were circulating in Iranian poultry sector. This concern is always present as M segment might be introduced into human influenza viruses by reassortment phenomenon. | ||
| کلیدواژهها | ||
| adamantane drug resistance؛ Avian influenza virus؛ H9N2؛ M2 protein؛ Poultry | ||
| مراجع | ||
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