Malekan, Mohammad, VasfiMarandi, Mehdi, Barin, abas, Mokhtari azad, Talat, Ranjbar, Mohammad Mehdi, Bashashati, Mohsen. (1395). Molecular evaluation of M2 protein of Iranian avian influenza viruses of H9N2 subtype in order to find mutations of adamantane drug resistance. , 10(4), 253-262. doi: 10.22059/ijvm.2016.59718
Mohammad Malekan; Mehdi VasfiMarandi; abas Barin; Talat Mokhtari azad; Mohammad Mehdi Ranjbar; Mohsen Bashashati. "Molecular evaluation of M2 protein of Iranian avian influenza viruses of H9N2 subtype in order to find mutations of adamantane drug resistance". , 10, 4, 1395, 253-262. doi: 10.22059/ijvm.2016.59718
Malekan, Mohammad, VasfiMarandi, Mehdi, Barin, abas, Mokhtari azad, Talat, Ranjbar, Mohammad Mehdi, Bashashati, Mohsen. (1395). 'Molecular evaluation of M2 protein of Iranian avian influenza viruses of H9N2 subtype in order to find mutations of adamantane drug resistance', , 10(4), pp. 253-262. doi: 10.22059/ijvm.2016.59718
Malekan, Mohammad, VasfiMarandi, Mehdi, Barin, abas, Mokhtari azad, Talat, Ranjbar, Mohammad Mehdi, Bashashati, Mohsen. Molecular evaluation of M2 protein of Iranian avian influenza viruses of H9N2 subtype in order to find mutations of adamantane drug resistance. , 1395; 10(4): 253-262. doi: 10.22059/ijvm.2016.59718

Molecular evaluation of M2 protein of Iranian avian influenza viruses of H9N2 subtype in order to find mutations of adamantane drug resistance

Iranian Journal of Veterinary Medicine
مقاله 2، دوره 10، شماره 4، 2016، صفحه 253-262    اصل مقاله (1.48 M)
نوع مقاله: Infectious agents- Diseases
شناسه دیجیتال (DOI): 10.22059/ijvm.2016.59718
نویسندگان
Mohammad Malekan1؛ Mehdi VasfiMarandi* 2؛ abas Barin3؛ Talat Mokhtari azad4؛ Mohammad Mehdi Ranjbar3؛ Mohsen Bashashati5
11Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4Department of Virology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
5Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده
Background: The H9N2 subtype of influenza A viruses is considered to be widespread in poultry industry. Adamantane is a group of antiviral agents which is effective both in prevention and treatment of influenza A virus infections. These drugs inhibit M2 protein ion channel which has role on viral replication. OBJECTIVES: The main objective of this study is to evaluate M gene of avian influenza viruses (AIVs) of H9N2 subtype in order to find adamantane drug resistance mutations. METHODS: Over 100 suspected samples were collected from different geographical regions of Iran during 2012-2013. Samples were injected via allantoic sac of 9-11 day-old chicken embryos. A total of 11 out of 100 were AIV. The H9N2 subtype was confirmed by specific RT-PCR. The RT-PCR was conducted for full length M gene. PCR amplified products were purified and then conducted for commercial direct sequencing. Finally, sequences were checked for possible sites of adamantane resistance mutations. RESULTS: Overall, 8 out of 11 viruses harbored the adamantane resistance-associated mutations. Of which, four viruses were isolated in 2013 and four viruses in 2012. Two different resistance-associated mutations were observed during different years. CONCLUSIONS: The present study provided clear evidence concerning resistance AIVs of H9N2 subtype that were circulating in Iranian poultry sector. This concern is always present as M segment might be introduced into human influenza viruses by reassortment phenomenon.
کلیدواژه‌ها
adamantane drug resistance؛ Avian influenza virus؛ H9N2؛ M2 protein؛ Poultry
مراجع
آمار
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