Nazemi, Kamran, Ghalyanchi Langeroudi, Arash, Hashemzadeh, Masoud, Karimi, Vahid, Seger, Waleed, Ehsan, Mohammad Reza. (1395). Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene. , 10(4), 245-252. doi: 10.22059/ijvm.2016.59716
Kamran Nazemi; Arash Ghalyanchi Langeroudi; Masoud Hashemzadeh; Vahid Karimi; Waleed Seger; Mohammad Reza Ehsan. "Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene". , 10, 4, 1395, 245-252. doi: 10.22059/ijvm.2016.59716
Nazemi, Kamran, Ghalyanchi Langeroudi, Arash, Hashemzadeh, Masoud, Karimi, Vahid, Seger, Waleed, Ehsan, Mohammad Reza. (1395). 'Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene', , 10(4), pp. 245-252. doi: 10.22059/ijvm.2016.59716
Nazemi, Kamran, Ghalyanchi Langeroudi, Arash, Hashemzadeh, Masoud, Karimi, Vahid, Seger, Waleed, Ehsan, Mohammad Reza. Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene. , 1395; 10(4): 245-252. doi: 10.22059/ijvm.2016.59716

Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene

Iranian Journal of Veterinary Medicine
مقاله 1، دوره 10، شماره 4، 2016، صفحه 245-252    اصل مقاله (903.3 K)
نوع مقاله: Infectious agents- Diseases
شناسه دیجیتال (DOI): 10.22059/ijvm.2016.59716
نویسندگان
Kamran Nazemi1؛ Arash Ghalyanchi Langeroudi* 2؛ Masoud Hashemzadeh3؛ Vahid Karimi4؛ Waleed Seger1؛ Mohammad Reza Ehsan5
1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.
2Department of Microbiology and immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran - IRAN
3Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, IRAN.
4Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.
5Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده
Background: Avian infectious bronchitis (IB), with avian
infectious bronchitis virus (IBV) as the causing agent, is a ubiquitous endemic
disease of the chicken with devastating effects on its industry. A viral
membrane surface protein called S  not
only induces neutralizing antibodies but also plays an important role in virus
binding and entry to host cells. Technically, S1 protein gene sequencing also
helps greatly in IBV genotyping. OBJECTIVES: The aim of this study was
to characterize Iranian IBV based on S2 gene. METHODS: After RT-PCR
amplification, the S2 gene of nine Iranian IBV isolates were sequenced and then
compared with reference strains. RESULTS: The isolates were classified
into genotype I as Massachusetts like IB Vs, genotype VII which clustered into
two branches, VIIa (IS-1494 like IB viruses), and VIIb, and was related to QX-
like viruses and Genotype VIII as 793/B like IBVs. CONCLUSIONS: As far
as we know, this is the first S2-based classification study on Iranian IBV
isolates providing a firm experimental basis to correlate with genotypic
characterization
کلیدواژه‌ها
Characterization؛ infectious bronchitis virus؛ genotyping؛ Phylogenetic study؛ spike
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