پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 158 |
| تعداد شماره‌ها | 6,225 |
| تعداد مقالات | 67,685 |
| تعداد مشاهده مقاله | 114,983,652 |
| تعداد دریافت فایل اصل مقاله | 89,655,361 |
ریزازدیادی ختمی چینی در شرایط درون شیشه ای | ||
| به نژادی گیاهان زراعی و باغی | ||
| مقاله 5، دوره 3، شماره 2، آذر 1394، صفحه 191-200 اصل مقاله (3.47 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| نویسندگان | ||
| فاطمه فیضی1؛ موسی موسوی* 2؛ مهر انگیز چهرازی2 | ||
| 1کارشناس ارشد باغبانی (گل و گیاهان زینتی) گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز | ||
| 2استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز | ||
| چکیده | ||
| به منظور بررسی ریزازدیادی ختمی چینی در شرایط درونشیشهای، سه آزمایش به صورت طرح کاملاً تصادفی با 10 تکرار، در آزمایشگاه کشت بافت دانشکده کشاورزی دانشگاه شهید چمران اهواز در سال 1393 انجام گرفت. در آزمایش اول محیط پایه مناسب مشخص شد. آزمایش دوم بهترین غلظت هورمون BAP جهت القای شاخه و آزمایش سوم بهترین نوع و غلظت هورمون ریشهزایی مشخص شد. مقایسه میانگین آزمایش اول نشان داد که بیشترین میانگین طول شاخساره، تعداد برگ، وزن تر و وزن خشک شاخساره در محیط VS نسبت به سایر محیطها مشاهده شد. مقایسه میانگین آزمایش دوم نشان داد که غلظت 5/0 میلیگرم بر لیتر هورمون BAP بیشترین میانگین طول شاخساره، تعداد برگ، وزن تر و وزن خشک شاخساره نسبت به سایر غلظتها را داشت. مقایسه میانگین آزمایش سوم نشان داد که غلظت 2/0 میلیگرم در لیتر هورمون IBAبیشترین میانگین درصد ریشهزایی، تعداد ریشه و طول ریشه نسبت به سایر غلظتها را داشت. | ||
| کلیدواژه‌ها | ||
| بنزیل آمینوپورین؛ پزآوری؛ ختمی چینی؛ ریشه زایی؛ محیط VS | ||
| عنوان مقاله [English] | ||
| Micropropagation of Hibiscus rosa-sinensis through tissue culture | ||
| نویسندگان [English] | ||
| Fatemeh Feizi1؛ Mousa Mousavi2؛ Mehrangiz Chehrazi2 | ||
| 1Graduate M.Sc. Student, Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz. Ahvaz | ||
| 2Assistant Professor, Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz | ||
| چکیده [English] | ||
| In order to investigate the in vitro micropropagation Hibiscus Rosa-sinensis three tests in a completely randomized design with 10 replications were performed in tissue culture laboratory Shahid Chamran University in 2014.The first experiment showed the perfect media.Second tests best consentration BAP for multiplication and third tests showed the best type and concentration rooting hormone. Compare average of first experiments showedthe highest average shoot length, number of leaves, fresh weight and dry weight was observed in VS than other media. Compare average of second experiments showedthe concentration of 5.0 mg per liter hormone BAP highest average shoot length, number of leaves, fresh weight and dry weight than other concentrations was observed.Compare third experiment showed that the average concentration of 2.0 mg per liter of IBA hormone highest percentage of rooting, number of roots and root length was compared to other concentrations. | ||
| کلیدواژه‌ها [English] | ||
| BAP, Hibiscus rosa-sinensis, Media VS, proliferation, Rotting | ||
| مراجع | ||
|
1 . Andreu JS, Jorda J and Juarez M (1991) Reactions of FeEDTA and FeEDDHA applied to calcareous soils. In Iron nutrition and interactions in plants. Springer Netherlands. Pp. 57-62. 2 . Azadi P, Khosh-Khui M, Beyramizadeh E and Bagheri H (2007) Optimization of Factors Affecting in vitro Proliferation and Rooting of Rosa hybrida L. cv.‘Rafaela’. International Journal of Agricultural Research. 2(7): 626-631. 3 . Bayanati M and Mortazavi SN (2013) Mass propagation of Rosa hybrid cv. Black baccara by periodical bioreactor. International Journal of Agriculture: Research and Review. 3(2): 241-245. 4 . Carpenter WJ and Cornell JA (1992) Auxin application duration and concentration govern rooting of Hibiscus stem cuttings. Journal of the American Society for Horticultural Science. 117(1): 68-74. 5 . Castillo B, Smith MAL, Madhavi DL and Yadava UL (1997) Interactions of irradiance level and iron chelate source during shoot tip culture of Carica papaya L. HortScience. 32(6): 1120-1123. 6 . Christensen B, Sriskandarajah S, Serek M and Muller R (2008) In vitro culture of Hibiscus rosa-sinensis L.: influence of iron, calcium and BAP on establishment and multiplication. Plant Cell, Tissue and Organ Culture. 93(2): 151-161. 7 . Cos JD, Frutos R and Garcia J (2004) In vitro rooting study of the peach-almond hybrid GF677. Acta Horticulture. 658: 623-627. 8 . Dimassi-Theriou K (1994) In vitro propagation of cv. Kalamon olives (Olea europaea L.). Advances Horticulture. 8: 185-189. 9 . Ghaffar FRA and El-Elaimy IA (2012) In vitro, antioxidant and scavenging activities of Hibiscus rosa sinensis crude extract. Journal of Applied Pharmaceutical Science. 2(01): 51-58. 10 . George EF (2008) Plant propagation by tissue culture. 3th Edition, (eds.), 175-281. Springer. 11 . Govinden-Soulange J, Boodia N, Dussooa C, Gunowa R, Deensah S, Facknath S and Rajkomar B (2009) Vegetative propagation and tissue culture regeneration of Hibiscus sabdariffa L. (Roselle).World Journal of Agricultural Sciences. 5(5): 651-661. 12 . Khaleghi A, Sahraroo A, Rasoulnia IN and Ataei R (2008) In vitro propagartion of Alstromeria cv. Fuego. American Eurasian Journal of Agriculture and Environmental Science. 3: 492-497. 13 . Khosravi P, Jafarkhani Kermani M, Nematzadeh GA and Bihamta MR (2007) A protocol for mass production of Rosa hybrida cv. Iceberg through in vitro propagation. Iranian Journal of Biotechnology. 5(2): 100-104. 14 . Lambardi M and Rugini E (2003) Micro Propagation of Olive (Olea europaea L.). In: MicroPropagation of Woody Trees and Fruits. Kluwer Academic Publisher Netherland, Editor: Daniel Mohan Bienstock, Katsuaki Ishii (Editor), ISBN-13:9781402011351. Pp. 621-646. 15. Lucena JJ, Manzanares M and Garate A (1992) A test to evaluate the efficacy of commercial Fe‐chelates. Journal of Plant Nutrition. 15(10): 1553-1566. 16 . Lucena JJ (2003) Fe chelates for remediation of Fe chlorosis in strategy I plants. Journal of Plant Nutrition. 26(10-11): 1969-1984. 17 . Marschner H (1995) Functions of mineral nutrients: macronutrients. Mineral Nutrition of Higher Plants. 87(2): 379-396. 18 . Rout GR, Mohapatra A and Jain SM (2006) Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnology Advances. 24(6): 531-560. 19 . Shibli RA, Mohammad MJ and Ajlouni ZI (2002) Growth and micronutrient acquisition of in vitro grown bitter almond and sour orange in response to iron concentration from different iron chelates. Journal Plant Nutrition. 25: 1599-1606. 20 . Singh SK, Misra RL and Ranjan JK (2012) In vitro shoot regeneration from cormel derived callus of gladiolus and bio-hardening of plantlets. Indian Journal of Biotechnology. 11(1): 99-104. 21 . Skirvin RM, Chu MC and Young HJ (1990) Rose. Handbook of plant cell culture. 96(5): 716-743. 22 . Torkashvand AM and Shadparvar V (2012) Rooting in hibiscus rosa-sinesis (Yellow doublehybrid) by indole butyric acid and rooting substrates. International Journal of Plant, Animal and Environmental Sciences. 9(3): 194-198. 23 . Torres KC (1989) Tissue culture techniques for Horticulturel Crops. Published by Van, No. Strand Reinhold, NewYork. 88(4): 100-107. 24 . Xing W, Bao M, Qin H and Ning G (2010) Micropropagation of Rosa rugosa through axillary shoot proliferation. Acta Biologica Cracoviensia Series Botanica. 52(2): 69-75. 25 . Van der Salm TP, Van der Toorn CJ, Ten Cate CHH, Dubois LA, De Vries DP and Dons HJ (1994) Importance of the iron chelate formula for micropropagation of Rosa hybrida L.‘Moneyway’. Plant Cell, Tissue and Organ Culture. 37(1): 73-77. 26 . Vijaya N and Satyanarayana G (1991) Effect of culture media and growth regulators on in vitro propagation of rose. In Horticulture - New technologies and applications. Springer Netherlands. Pp. 209-214.
| ||
|
آمار تعداد مشاهده مقاله: 2,122 تعداد دریافت فایل اصل مقاله: 1,026 |
||