پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 158 |
| تعداد شماره‌ها | 6,228 |
| تعداد مقالات | 67,750 |
| تعداد مشاهده مقاله | 115,079,019 |
| تعداد دریافت فایل اصل مقاله | 89,816,409 |
بررسی فراوانی حامل‌های ویروس تب برفکی در گوسفندان ذبحی کشتارگاه صنعتی مشهد با استفاده از RT-PCR | ||
| مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
| مقاله 2، دوره 70، شماره 1، فروردین 1394، صفحه 7-14 اصل مقاله (268.81 K) | ||
| شناسه دیجیتال (DOI): 10.22059/jvr.2015.52963 | ||
| نویسندگان | ||
| سعید زیبائی* 1؛ صمد رضائی2؛ محمد رشتی باف3 | ||
| 1موسسه تحقیقات واکسن و سرم سازی رازی شعبه شمال شرق، بخش تحقیقات دامپزشکی و بیوتکنولوژی، مشهد–ایران | ||
| 2دانش آموخته گروه بیوشیمی، دانشگاه پیام نور مشهد، مشهد –ایران | ||
| 3بخش بررسی‌ها، اداره کل دامپزشکی استان خراسان رضوی، مشهد –ایران | ||
| چکیده | ||
| زمینه مطالعه: بیماری تب برفکی در ایران بومی می‌باشد. روش‌های تشخیصی مولکولی توان بالقوه مهمی برای تشخیص دام‌هایی که دارای عفونت پنهان بوده و یا حامل میباشند، ارائه نموده است. هدف: بررسی فراوانی حامل‌های ویروس تب برفکی در گوسفندان ذبحی کشتارگاه صنعتی مشهد با استفاده از RT-PCR. روش کار: پس از جداسازی 94نمونه از لوزه گوسفندان، واکنش RT-PCR برای تشخیص تب برفکی و تعیین سروتیپ ویروس تب برفکی انجام گردید و در انتها تعیین توالی اسید نوکلئیک انجام شد. نتایج: نتایج به دست آمده از تشخیص ویروس تب برفکی نشان داد 23 نمونه (5/24 )% از نظر وجود RNA ویروس تب برفکی مثبت میباشند که از این تعداد 20 نمونه تیپ O تشخیص داده شد و 3 نمونه از نمونه‌های مثبت از نظر تب برفکی به تعیین تیپ با پرایمر‌های اختصاصی جواب ندادند، همچنین نتایج حاصل تعیین توالی اسید نوکلئیک ناحیه D1 ژنــوم ویــروس نـشــان داد کـه ویـروس تـب بـرفکـی حـامـل گـوسفنـدی Sheep)2010/100(O/IRN/ دارای 02/92% تشـابـه بـا ویـروس 2005-2001/67O/IRN/ و42/88% تشابه با ویروس 2008-2004/15O/IRN/ می‌باشد. نتیجه‌گیری نهایی: مطالعه حاضر نشان داد که در صـد گـوسـفـنـدان حـاملـیـن ویـروس تـب بـرفکی در کشتارگاه مشهد قابل توجه می‌باشد. توصیه می‌گردد تا ازبر آورد دوره‌ای حاملین در گاو و نشخوارکنندگان کوچک بعنوان آگاهی دهنده از برنامه‌های کنترلی در منطقه استفاده گردد. | ||
| کلیدواژه‌ها | ||
| ویروس تب برفکی؛ شناسایی مولکولی؛ حاملین گوسفندی | ||
| عنوان مقاله [English] | ||
| Evaluate the frequency of foot and mouth disease viral carriers in slaughtered sheep in Mashhad industrial abattoir using RT-PCR | ||
| نویسندگان [English] | ||
| Saeid Zibaee1؛ Samad Rezaee2؛ Mohammad Rashtibaf3 | ||
| 1Department of Veterinary Research and Biothecnology, Razi vaccine and Serum Research Institute, North East Branch, Mashhad-Iran | ||
| 2Graduated from the Payam-e-Noor University of Mashhad, Department of Biochemistry, Mashhad-Iran | ||
| 3Department of Investigation, khorasan-Razavi Provincial Veterinary Service, Mashhad-Iran | ||
| چکیده [English] | ||
| BACKGROUND: Foot -and- mouth disease (FMD) is endemic in Iran. Molecular techniques for diagnosis of persistent infection or carrier animals have shown a potential ability to improve the detection of a low genome copy number in samples. Objectives: The purpose of this study is to evaluate the frequency of foot and mouth disease viral carriers in slaughtered sheep in Mashhad industrial abattoir using RT-PCR. Methods: Samples were isolated from tonsil of 94 slaughtered sheep analyzed by RT-PCR experiment for the detection of FMD, identification of FMD virus serotypes and at the end nucleic acid sequencing were performed. Results: The results showed that the 23 samples (24/5 percent) were positive for the presence of FMD virus RNA, of which 89.9% of cases are type O and 3 cases of FMD samples did not respond. The results of the 1D genome sequencing of the nucleic acid virus showed that FMD virus of sheep (O/IRN/100/2010Sheep), has 92/02% similarity with the virus (O/IRN/67/2001-2005) and 88/42% similarity with the virus (O/IRN/15/2004-2008). Conclusions: This study showed that the percentage of FMDV sheep carriers in Mashhad slaughterhouse was remarkable. Estimation of the frequency of carrier state in cattle and small ruminants is recommended as a monitor of control plan in the region. | ||
| کلیدواژه‌ها [English] | ||
| FMD virus, Molecular detection, sheep carriers | ||
| مراجع | ||
|
Alexanderson, S., Zhang, Z., Donaldson, A.I., Garland, A.J.M. (2003) The Pathogenesis and diagnosis of Foot-and-Mouth Disease. J Comp Pathol. 129: 1-36.
Alexanderson, S., Zhang, Z., Donaldson, A.I. (2002) Aspect of the persistence of foot-and-mouth disease virus in animals, the carrier problem. Microbes Infect. 4: 1099-1110.
Bahari, A., Taghipour-Bazargani, T., Marquardt, O., Ghorashi, S.A., Bokaie, S. (2007) Application of nested-PCR for detection of foot and- mouth disease viral sequences in tonsil of slaughtered cattle with clinically normal appearance in Iran. Vet Arh. 77: 299-306.
Blancou, J. (2002) History of control of footand- mouth disease. Comp Immunol Microb. 25: 283-293.
Callens, M., De Clercq, K., Gruia, M., Danes, M. (1998) Detection of foot-and-mouth disease by reverse transcription polymerase chain reaction and virus isolation in contact sheep without clinical signs of foot-and-mouth disease. Vet Q. 20: 37- 40.
Domingo, E. (1998) Quasispecies and implications for virus persistence and escape. Clin Diagn Virol. 10: 97-101.
Fenner, F., Bachmann, P.A., Gibbs, E.P.J., Murphy, F.A., Studdert, M.J., White, D.O. (1993) Veterinary Virology, (2nd ed.) Academic Press, INC, California, USA.
Ghorashi, S.A., Hagian, T., Daliri, M., Banuei, M.M., Alvand, A.R. (2002) Detection of foot-and-mouth disease virus in clinical samples by RT-PCR. Pajouhesh-Va-sazandegi (In Persian). 53: 10-12.
Gurhan, S.I., Gurhan, B., Osturkmen, A., Aynagoz, G., Candas, A., Kizil S. (1993) Establishment of the prevalence of persistently infected cattle and sheep in Anatolia with FMDV. J Etlik Vet Microbiol. 7: 52-59.
Hedger, R.S. (1968) The isolation and characteriz-ation of foot-and-mouth disease from clinically normal herds of cattle in Botswana. J Hyg (Lond). 66: 27-36.
Hughes, G.J., Mioulet, V., Haydon, D.T., Kitching, R, P., Donaldson, A.I., Woolhouse, M.E.J. (2002) Serial passage of foot-and-mouth disease virus in sheep reveals declining levels of viraemia over time. J Gen Virol. 83: 1907-1914.
Kitching, R.P. (2002) Identification of foot and- mouth disease virus carrier and subclinically infected animals and differentiation from vaccinated animals. Rev Sci Tech Oie. 21: 531- 538.
Kitching, R.P. (2002) Clinical variation in foot and mouth disease: cattle. Rev Sci Tech Off Int Epiz. 21: 499-504.
Knowles, N.J., Samuel, A.R. (1998) RT-PCR and Sequencing Protocols for the Molecular Epidemio-logy of Exotic Virus Diseases of Animals. (1st ed.). Institute for Animal Health. Pirbright, UK.
Loth, L., Osmani, M.G., Kalam, M.A., Chakraborty, R.K., Wadworth, J., Knowles, N.J., Hammond, J.M., Benigno, C. (2011) Molecular characterization of Foot- and - mouth disease virus: implication for disease control in Bangeladesh. Transbound Emerg Dis. 58: 240-246.
Murphy, F.A., Gibbs, E.P.J., Horzinek, M.C., Studdert, M.J. (1999) Veterinary Virology. (3rd ed.) Academic Press. San Diego, California, USA.
OIE. (2002) Foot and Mouth Disease: Facing the New Dilemmas; volume 21. (11th ed.) OIE, Paris, France.
Prato Murphy, M.L., Meyer, R.F., Mebus, C., Schudel, A.A., Rodriguez, M. (1994) Analysis of sites of foot-and-mouth disease virus persistence in carrier cattle via the polymerase chain reaction. Arch Virol. 136: 299-307.
Rashtibaf, M., Sharifi, K., Zibaee, S., Dehghani, H. (2012) A survey on the frequency of foot-and-mouth disease virus carriers in cattle in north-east of Iran by RT-PCR. Transbound Emerg Dis. 59: 482-489.
Rasooli, A., Seyfi abad Shapouri, M.R., Haji Hajikolaei, M.R., Komeilian, A. (2013) Search for FMD virus carrying buffaloes slaughtered in Ahvaz abattoir. Ir Vet J. 8: 28-35.
Reid, S.M., Forsythm, M.A., Hutchings, G.H., Ferriis, N.P. (1998)Comparison of reverse trans-cription polymerase chain reaction , enzyme linked mmunosorbent assay and virus isolation for the routine diagnosis of foot- and- mouth disease. J Virol Method. 70: 213-217.
Reid, S.M., Ferris, N.P., Hutchings, G.H., Samuel, A.R., Knowles, N.J. (2000) Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction. J Virol Method. 89: 167-176.
Reid, S.M., Ferris, N.P., Hutching, G.H., De Clerq, K., Newman, B.J., Knowles, N.J., Samuel, A.R. (2001) Diagnosis of foot-and-mouth disease by RT-PCR: use of phylogenetic data to evaluate primers for typing of viral RNA in clinical samples. Arch Virol. 146: 2421-2434.
Salt, J.S. (1993) The carrier satate in foot and mouth disease-An immunological review. Br Vet J. 149: 207-223.
Samuel, A.R., Knowles, N.J., Kitching, R.P. (1988). Serological and biochemical analysis of some recent type A foot-and-mouth disease virus from the middle East. Epidemiol Infect. 101: 577-590.
Sutmoller, P., Barteling, S.S., Olascoaga, R.C., Sumption, K.J. (2003) Control and eradication of foot-and-mouth disease. Virus Res. 91: 101-144.
Taleb shooshtari, A. (1996) FMD and its status in IR Iran. Pajouhesh-Va-sazandegi (In Persian). 29: 82-85.
Vangrysperre, W., De Clerq, K. (1996) Rapid and sensitive polymerase chain reaction based detection and typing of foot-and-mouth disease virus in clinical samples and cell culture isolates, combined with a simultaneous differentiation with other genomically and/or symptomatically related viruses. Arch Virol. 141: 331-344.
Vallat, B. (2002) Role of the international organiz-ation for animal health (Office International des Epizooties: OIE) in the control of foot-and-mouth disease. Comp immunol microb. 25: 383-392.
Zhang, Z., Alexanderson, S. (2003) Detection of carrier cattle and sheep persistently infected with foot-and-mouth disease virus by a rapid real-time RT- PCR assay. J Virol Method. 111: 95-100.
Zhang, Z.D., Kitching, R.P. (2001) The localization of persistent foot and mouth disease virus in the epithelial cells of the soft palate and pharynx. J Comp Pathol. 124: 89-94.
Zibaei, S., Keivanfar, H., Rabbani, M., Hematzadeh, F., Kianizade, M., Fathi najafi, M. (2010) Comparative studyon 1d (vp1) region offoot and mouth disease virus (type astrain) amongdif-ferentisolates: khorasan razavi isolate and other iranian and neighboring countries isolates. J Vet Res (University of Tehran). 65: 199-202.
Zibaei, S., Keivanfar, H., Rabbani, M., Kianizade, M., Hematzadeh, F., Bokaie, S. (2007) Identification of the foot and mouth disease foci from susceptible foci in khorasan razavi province. J Vet Res (University of Tehran). 62:151-155. | ||
|
آمار تعداد مشاهده مقاله: 1,766 تعداد دریافت فایل اصل مقاله: 1,012 |
||