تعداد نشریات | 161 |
تعداد شمارهها | 6,562 |
تعداد مقالات | 70,812 |
تعداد مشاهده مقاله | 124,906,892 |
تعداد دریافت فایل اصل مقاله | 98,135,097 |
Intestinal colonization of different Brachyspira spp. in laying hens | ||
Iranian Journal of Veterinary Medicine | ||
مقاله 9، دوره 8، شماره 3، دی 2014، صفحه 213-218 اصل مقاله (118.29 K) | ||
شناسه دیجیتال (DOI): 10.22059/ijvm.2014.51894 | ||
نویسندگان | ||
Mina Zarabi1؛ Abdollah Jamshidi* 2؛ ُSaeid Khanzadi2؛ Jamshid Razmyar3 | ||
1Graduated from the Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
2Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
3Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
چکیده | ||
BACKGROUND:Avian intestinal spirochetosis (AIS) is caused by spiral-shaped Gram-negative Brachyspira spp. in poultry. It is known as a cause of diarrhea, low egg production, and increased occurrence of dirty eggs in layer hens. OBJECTIVES: In this study, the presence of some Brachyspira spp. was investigated in laying hens. METHODS: A total of 100 cloacal swab samples were individually collected from 20 laying hen flocks showing fecal egg staining in northeast of Iran. RESULTS: Using culture and morphologic examination, 41 samples (41%) from 20 flocks were positive; however, by using genus-specific PCR, only 37 (37%) samples were confirmed as Brachyspira spp. Using speciesspecific primers, single colonization was identified in 18 samples associated with B. pilosicoli (48.6%), while single colonization with B. intermedia was found in only two samples (5.4%). Simultaneous colonization by B. intermedia and B. murdochii was detected in 3 samples (8.1%). B. pilosicoli was the most prevalent species in concurrent colonization in 11 cases (29.7%). Finally, cocolonization by B. intermedia and B. innocens was identified in 3 samples (8.1%). CONCLUSIONS: The results of this study showed the colonization of different species of Brachyspira with dominance of B. pilosicoli in layer hens. | ||
کلیدواژهها | ||
Brachyspira؛ intestinal spirochetosis؛ Laying Hen؛ PCR | ||
آمار تعداد مشاهده مقاله: 2,348 تعداد دریافت فایل اصل مقاله: 1,475 |