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Mirmasoumi, Masoud, Azadi, Pejman, Sharafi, Ali, Ntui Otang, Valentine, Mii, Masahiro. (1392). Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer. , 3(2), 117-122. doi: 10.22059/pbs.2013.35828
Masoud Mirmasoumi; Pejman Azadi; Ali Sharafi; Valentine Ntui Otang; Masahiro Mii. "Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer". , 3, 2, 1392, 117-122. doi: 10.22059/pbs.2013.35828
Mirmasoumi, Masoud, Azadi, Pejman, Sharafi, Ali, Ntui Otang, Valentine, Mii, Masahiro. (1392). 'Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer', , 3(2), pp. 117-122. doi: 10.22059/pbs.2013.35828
Mirmasoumi, Masoud, Azadi, Pejman, Sharafi, Ali, Ntui Otang, Valentine, Mii, Masahiro. Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer. , 1392; 3(2): 117-122. doi: 10.22059/pbs.2013.35828

Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer

Progress in Biological Sciences
مقاله 8، دوره 3، شماره 2، اسفند 2013، صفحه 117-122    اصل مقاله (756.96 K)
نوع مقاله: Original Research Papers
شناسه دیجیتال (DOI): 10.22059/pbs.2013.35828
نویسندگان
Masoud Mirmasoumi1؛ Pejman Azadi* 2؛ Ali Sharafi3؛ Valentine Ntui Otang4؛ Masahiro Mii4
1Department of Botany, School of Biology, Collage of Science, University of Tehran, Tehran, Iran
2Tissue Culture and Genetic Engineering Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
3Novin Giti Gene (NGG) Co., Bio-Incubator Center NIGEB, Tehran, Iran
4Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City, Chiba 271-8510, Japan
چکیده
Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased the organogenesis response and produced 4.4 bulblets per explants. Two months later, the bulblets were transferred to MS PGRs-free medium. Bulblets were successfully transplanted to the soil after a cold treatment of 8 weeks,
with a survival rate of 85%.
کلیدواژه‌ها
Lilium؛ Auxin؛ organogenesis؛ Regeneration
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