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Mohyedini, Shahab-aldin, Jamshidi, Shahram, Rafati, Sima, Nikbakht Boroujeni, Gholam Reza, Malmasi, Abdolali, Taslimi, Yasaman, Akbarein, Hesam-aldin. (1392). Comparison of immunochromatographic rapid test with molecular method in diagnosis of canine parvovirus. , 7(1), 57-61. doi: 10.22059/ijvm.2013.32024
Shahab-aldin Mohyedini; Shahram Jamshidi; Sima Rafati; Gholam Reza Nikbakht Boroujeni; Abdolali Malmasi; Yasaman Taslimi; Hesam-aldin Akbarein. "Comparison of immunochromatographic rapid test with molecular method in diagnosis of canine parvovirus". , 7, 1, 1392, 57-61. doi: 10.22059/ijvm.2013.32024
Mohyedini, Shahab-aldin, Jamshidi, Shahram, Rafati, Sima, Nikbakht Boroujeni, Gholam Reza, Malmasi, Abdolali, Taslimi, Yasaman, Akbarein, Hesam-aldin. (1392). 'Comparison of immunochromatographic rapid test with molecular method in diagnosis of canine parvovirus', , 7(1), pp. 57-61. doi: 10.22059/ijvm.2013.32024
Mohyedini, Shahab-aldin, Jamshidi, Shahram, Rafati, Sima, Nikbakht Boroujeni, Gholam Reza, Malmasi, Abdolali, Taslimi, Yasaman, Akbarein, Hesam-aldin. Comparison of immunochromatographic rapid test with molecular method in diagnosis of canine parvovirus. , 1392; 7(1): 57-61. doi: 10.22059/ijvm.2013.32024

Comparison of immunochromatographic rapid test with molecular method in diagnosis of canine parvovirus

Iranian Journal of Veterinary Medicine
مقاله 8، دوره 7، شماره 1، تیر 2013، صفحه 57-61    اصل مقاله (138.34 K)
شناسه دیجیتال (DOI): 10.22059/ijvm.2013.32024
نویسندگان
Shahab-aldin Mohyedini1؛ Shahram Jamshidi* 1؛ Sima Rafati2؛ Gholam Reza Nikbakht Boroujeni3؛ Abdolali Malmasi1؛ Yasaman Taslimi2؛ Hesam-aldin Akbarein4
1Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Molecullar Immunology and Vaccine Research Laboratory, Pasteur Institute of Iran, Tehran, Iran
3Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده
BACKGROUND: Canine parvovirus (CPV) infection is one of
the most common causes of infectious gastroenteritis in dogs and
is a highly contagious, often fatal disease. The original virus
(CPV type 2) has had some mutations since its emergence and
new variants (CPV-2a, 2b and 2c) have been reported from many
countries all around the world. Early diagnosis and treatment can
profoundly affect the disease outcome. OBJECTIVES:To compare
the ability of Immunochromatographic (IC) test to detect CPV
infection in 50 PCR positive samples (n=50) with regard to virus
strains. METHODS: 50 rectal swabs (n=50) were prepared from
suspicious dogs and subjected to PCR and IC test respectively.
RESULTS: The sensitivity of IC test in PCR positive samples was
84% (42 out of 50 samples) and the positive predictive value of
the test was 100%. Using PCR, CPV strains in our study were 2a
(18/50, 36%) and 2b (32/50, 64%) with the predominance of 2b
strain. IC test was also able to diagnose 15/18 (83.3%) of CPV-
2a and 27/32 (84.3%) CPV-2b strain positive samples, which
means IC test can detect CPV infections caused by both virus
strains (2a and 2b), without significant difference. CONCLUSIONS:
This study shows that IC test results are relatively reliable for
diagnosing CPVinfection in daily veterinary practice and the test
is able to diagnose both CPV-2a and CPV-2b which are prevalent
strains in Iran.
کلیدواژه‌ها
canine parvovirus؛ detection methods؛ PCR؛ immunochromatography
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