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. (1374). INTERACTION OF DOXORUBICIN WITH DNA-HMG1 COMPLEX. , 6(3), -.
. "INTERACTION OF DOXORUBICIN WITH DNA-HMG1 COMPLEX". , 6, 3, 1374, -.
. (1374). 'INTERACTION OF DOXORUBICIN WITH DNA-HMG1 COMPLEX', , 6(3), pp. -.
. INTERACTION OF DOXORUBICIN WITH DNA-HMG1 COMPLEX. , 1374; 6(3): -.

INTERACTION OF DOXORUBICIN WITH DNA-HMG1 COMPLEX

Journal of Sciences, Islamic Republic of Iran
مقاله 4، دوره 6، شماره 3، آبان 1995    اصل مقاله (512.53 K)
چکیده
In this study, the interaction of the anthracycline antibiotic doxorubicin
with DNA-HMG 1 complex was investigated employing UV/VIS spectroscopy,
thermal denaturation and DNA cellulose chromatography techniques. The
results indicated that the binding of doxorubicin to the protein reduces its Tm
in a dose dependent manner. Although doxorubicin protects free DNA
against thermal denaturation in the presence of HMG 1, thermal melts represent
biphasic profile corresponding to DNA- drug, HMG1- drug and free
components. Interaction of antibiotic with DNA - HMGl complex using
DNA cellulose columns also implies that the drug binds strongly to singlestranded
DNA-HMG 1 complex rather than to double- stranded DNA-HMG 1
columns. The results suggest destabilization of HMGl protein by drug action
thereby preventing DNA-HMG 1 complex formation
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