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کوهی, محمد کاظم, Zayerzadeh, Ehsan, Eslami, Mohsen, Zadeh Hashem, Elham. (1391). Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line. , 6(2), 79-87. doi: 10.22059/ijvm.2012.28951
محمد کاظم کوهی; Ehsan Zayerzadeh; Mohsen Eslami; Elham Zadeh Hashem. "Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line". , 6, 2, 1391, 79-87. doi: 10.22059/ijvm.2012.28951
کوهی, محمد کاظم, Zayerzadeh, Ehsan, Eslami, Mohsen, Zadeh Hashem, Elham. (1391). 'Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line', , 6(2), pp. 79-87. doi: 10.22059/ijvm.2012.28951
کوهی, محمد کاظم, Zayerzadeh, Ehsan, Eslami, Mohsen, Zadeh Hashem, Elham. Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line. , 1391; 6(2): 79-87. doi: 10.22059/ijvm.2012.28951

Transcriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line

Iranian Journal of Veterinary Medicine
مقاله 3، دوره 6، شماره 2 - شماره پیاپی 1177763، شهریور 2012، صفحه 79-87    اصل مقاله (1.53 M)
شناسه دیجیتال (DOI): 10.22059/ijvm.2012.28951
نویسندگان
محمد کاظم کوهی1؛ Ehsan Zayerzadeh2؛ Mohsen Eslami3؛ Elham Zadeh Hashem1
1گروه علوم پایه، بخش سم شناسی، دانشکده دامپزشکی دانشگاه تهران
2بخش تحقیقات و تولید واکسن‌های باکتری‌های بی هوازی، موسسه تحقیقاتی واکسن و سرم سازی رازی
3گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه تهران
چکیده
BACKGROUND: Some of metal ions as environmental pollutants show estrogenic activity. This xenostrogenic compounds can be caused carcinogenicity in organs. The mechanism of carcinogenicity of metal ions is not clarified. OBJECTIVES: In this study, we investigated the Transcriptional effects of variety of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter by estrogen receptor a in MDA-MB 231 breast cancer cell line. METHODS: Cells were plated into flask (75cm2) at 1.3 density or into 12- well plates (Nunc) at a density of 100000 cells per well and were transfected with a total of 3 µg of plasmid DNA using calcium phosphate coprecipitation. Oestrogen and some metal ions were used for stimulation of transfected cells. RESULTS: Our results showed that copper and cadmium ions activating specifically the oxytocin promoter, and cobalt and possibly, mercury ions activating specifically the ERE-controlled promoter and the majority of the ions did not affect transcriptional activation significantly. CONCLUSIONS: The study revealed that some metal ions show estrogenic activity by classical or non-classical mechanisms as well as some metal ions exhibit estrogenic activity by undetermined mechanisms in transfected MDA-MB 231 cell line.
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