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Nikbakht Brujeni, Gholam Reza, Tabaabaei, Saeid, Khormali, Mahmood, Ashrafi, Iraj. (1388). Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins. , 3(1), -. doi: 10.22059/ijvm.2009.19607
Gholam Reza Nikbakht Brujeni; Saeid Tabaabaei; Mahmood Khormali; Iraj Ashrafi. "Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins". , 3, 1, 1388, -. doi: 10.22059/ijvm.2009.19607
Nikbakht Brujeni, Gholam Reza, Tabaabaei, Saeid, Khormali, Mahmood, Ashrafi, Iraj. (1388). 'Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins', , 3(1), pp. -. doi: 10.22059/ijvm.2009.19607
Nikbakht Brujeni, Gholam Reza, Tabaabaei, Saeid, Khormali, Mahmood, Ashrafi, Iraj. Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins. , 1388; 3(1): -. doi: 10.22059/ijvm.2009.19607

Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins

Iranian Journal of Veterinary Medicine
مقاله 6، دوره 3، شماره 1، مهر 2009    اصل مقاله (244.14 K)
شناسه دیجیتال (DOI): 10.22059/ijvm.2009.19607
نویسندگان
Gholam Reza Nikbakht Brujeni؛ Saeid Tabaabaei؛ Mahmood Khormali؛ Iraj Ashrafi
دانشکده دامپزشکی
چکیده
Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion, immunoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion and immunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of mammalian species. Anti-camel antibodies bound camel IgG1, IgG2, IgG3 subclasses like the subclasses of sheep, but no reactivity to all subclasses chains in cattle and horse antibodies were observed. Polyclonal anti-camel IgY was specifically light chain reactive. Overall, the potential of using egg yolk immunoglobulins as a convenient source of anti antibodies to camel immunoglobulins was demonstrated.
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