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Hematological Impacts of Gallic Acid, Disulfiram, and Dexamethasone in a Rat Model of LPS-Induced Sepsis | ||
| Iranian Journal of Veterinary Medicine | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 21 مرداد 1404 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2025.394171.1005787 | ||
| نویسندگان | ||
| Zahra Hojati1؛ Ali Rassouli* 2؛ Farhang Sasani3؛ jamileh Jsalar-Amoli4؛ Hamidreza Javadi5 | ||
| 1Deptartment of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 2Pharmacology Division, Deptartment of Comparative Biosciences, Faculty of Veterinary Medicine,University of Tehran,Tehran,Iran | ||
| 3Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 4Toxicology Department. Faculty of Veterinary Medicine, University of Tehran,Teran,Iran | ||
| 5Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Background: Sepsis is a life-threatening inflammatory condition associated with severe hematological disturbances. This study evaluated the protective effects of dexamethasone (DEX), disulfiram (DSF), and gallic acid (GA) against LPS-induced sepsis in rats. Methods: Thirty male Wistar rats (180-200 g) were divided into six groups (n=5): Control, LPS (10 mg/kg, a single intraperitoneal, i.p., injection), and four pretreatment groups receiving DEX (1 mg/kg/day, i.p., for 2 days), GA (200 mg/kg/day, orally, for 7 days), DSF (50 mg/kg/day, orally, for 3 days), or GA+DSF (200+50 mg/kg/day, orally, for 7 and 3 days, respectively). Three hours after of the last dose in pretreatment groups, LPS was administered, and blood samples were collected 20 hours post-LPS injection for hematological analysis. Results: LPS caused significant changes including: leukopenia (Mean difference: −4.99×10³/μL, p=0.002), neutrophilia (+6.31×10³/μL, p<0.0001), lymphopenia (−9.00×10³/μL, p<0.0001), thrombocytopenia (−702.8×10³/μL, p<0.0001), and increased highly significantly the neutrophil-to-lymphocyte (N/L) ratio (+10.2, 107 folds, p= 0.001). LPS also increased the red blood cell (RBC) count (+0.914×106/μL, p= 0.0063), hemoglobin (Hb) concentration (+2.04 g/dl, p= 0.0029), and hematocrit (HCT) levels (+9.02 %, p= 0.0004). DEX significantly ameliorated the LPS-induced leukopenia and thrombocytopenia (p≤0.0001) but exacerbated neutrophilia (p≤0.0001) and the N/L ratio (p≤0.05). DSF reduced the LPS-induced changes in RBC, Hb, and HCT levels (p≤0.001–0.0001) but had minimal effects on thrombocytopenia. GA showed limited influence on the LPS-induced hematological changes but modulated HCT (p≤0.01). The DSF-GA combination significantly decreased the LPS-induced changes on the Hb, HCT, and N/L levels (p≤0.05). Moreover, DSF and GA, alone or combined, demonstrated significant reduction in RBCs, neutrophils, N/L ratio, and HCT levels (p<05- p<0001) compared with DEX. Conclusion: The DSF+GA combination demonstrated good efficacy in mitigating sepsis-induced hematological disruptions compared to DEX by targeting inflammation through distinct mechanisms, offering a novel therapeutic approach in the management of sepsis. | ||
| کلیدواژه‌ها | ||
| Disulfiram؛ Gallic acid؛ Hematology؛ Neutrophil-to-lymphocyte ratio؛ Sepsis | ||
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آمار تعداد مشاهده مقاله: 37 |
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